PMID: 9537865Apr 16, 1998Paper

Effects of the nitric oxide synthase inhibitors N(G)-nitro-L-arginine methyl ester and aminoethyl-isothiourea on the liver microcirculation in rat endotoxemia

Journal of Hepatology
C O CorsoK Messmer

Abstract

The question whether nitric oxide protects or impairs organ perfusion during early endotoxemia has not been completely answered. To evaluate the regulative function of nitric oxide on organ microvascular perfusion and leukocyte accumulation during endotoxemia, we studied the influence of a non-selective nitric oxide inhibitor and a preferential inducible nitric oxide synthase inhibitor (respectively, N(G)-nitro-L-arginine methyl ester and aminoethyl-isothiourea) on liver microcirculation (intravital fluorescence microscopy) in a rat model. Two hours after intraportal injection of lipopolysaccharide (5 mg/kg in 10 min) the rats were randomly treated and received a bolus dose of N(G)-nitro-L-arginine methyl ester (10 mg/kg, n = 7), aminoethyl-isothiourea (10 mg/kg, n = 6) or normal saline, (n = 7). After 1 h, N(G)-nitro-L-arginine methyl ester blockade yielded a higher rate of non-perfused sinusoids than normal saline (27 +/- 2% vs 19 +/- 5%, p < 0.05). LPS-induced leukocyte stagnation in sinusoids was further increased (p < 0.05) in all groups after 1 h treatment, but N(G)-nitro-L-arginine methyl ester clearly accentuated leukocyte accumulation in sinusoids as compared to normal saline (69 +/- 19% vs 16 +/- 4%, p < 0.05). Both m...Continue Reading

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Citations

Dec 18, 2001·Pharmacology & Therapeutics·F FeihlL Liaudet
Aug 11, 2001·Free Radical Biology & Medicine·K Bian, F Murad
Sep 17, 2003·Critical Care : the Official Journal of the Critical Care Forum·Ryon M BatemanChristopher G Ellis
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Apr 29, 2008·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Stephen TrzeciakUNKNOWN Microcirculatory Alterations in Resuscitation and Shock (MARS) Investigators
Sep 27, 2012·Alcoholism, Clinical and Experimental Research·Jun-Ying ZhouAmin A Nanji
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Sep 13, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yoshiya ItoRobert S McCuskey
Jul 28, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Kalyani KarnamOnkar Prakash Kulkarni
Feb 15, 2011·Critical Care Nursing Clinics of North America·David J Lundy, Stephen Trzeciak

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