Effects of the novel mitochondrial protein mimitin in insulin-secreting cells

The Biochemical Journal
Katarzyna HanzelkaEwa Gurgul-Convey

Abstract

Mimitin, a novel mitochondrial protein, has been shown to act as a molecular chaperone for the mitochondrial complex I and to regulate ATP synthesis. During Type 1 diabetes development, pro-inflammatory cytokines induce mitochondrial damage in pancreatic β-cells, inhibit ATP synthesis and reduce glucose-induced insulin secretion. Mimitin was expressed in rat pancreatic islets including β-cells and decreased by cytokines. In the ob/ob mouse, a model of insulin resistance and obesity, mimitin expression was down-regulated in liver and brain, up-regulated in heart and kidney, but not affected in islets. To further analyse the impact of mimitin on β-cell function, two β-cell lines, one with a low (INS1E) and another with a higher (MIN6) mimitin expression were studied. Mimitin overexpression protected INS1E cells against cytokine-induced caspase 3 activation, mitochondrial membrane potential reduction and ATP production inhibition, independently from the NF-κB (nuclear factor κB)-iNOS (inducible NO synthase) pathway. Mimitin overexpression increased basal and glucose-induced insulin secretion and prevented cytokine-mediated suppression of insulin secretion. Mimitin knockdown in MIN6 cells had opposite effects to those observed afte...Continue Reading

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Citations

Jun 2, 2015·Biochemical and Biophysical Research Communications·Ewa Gurgul-ConveySigurd Lenzen
Oct 27, 2017·The Journal of Biological Chemistry·Claudine HahnEwa Gurgul-Convey
Mar 14, 2017·BioMed Research International·Nina Mickelson WeldinghAnette Kocbach Bølling
Jan 12, 2019·Cell Death & Disease·Karolina TykaEwa Gurgul-Convey
Aug 8, 2020·Cells·Ewa Gurgul-Convey

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