Abstract
Mimitin, a novel mitochondrial protein, has been shown to act as a molecular chaperone for the mitochondrial complex I and to regulate ATP synthesis. During Type 1 diabetes development, pro-inflammatory cytokines induce mitochondrial damage in pancreatic β-cells, inhibit ATP synthesis and reduce glucose-induced insulin secretion. Mimitin was expressed in rat pancreatic islets including β-cells and decreased by cytokines. In the ob/ob mouse, a model of insulin resistance and obesity, mimitin expression was down-regulated in liver and brain, up-regulated in heart and kidney, but not affected in islets. To further analyse the impact of mimitin on β-cell function, two β-cell lines, one with a low (INS1E) and another with a higher (MIN6) mimitin expression were studied. Mimitin overexpression protected INS1E cells against cytokine-induced caspase 3 activation, mitochondrial membrane potential reduction and ATP production inhibition, independently from the NF-κB (nuclear factor κB)-iNOS (inducible NO synthase) pathway. Mimitin overexpression increased basal and glucose-induced insulin secretion and prevented cytokine-mediated suppression of insulin secretion. Mimitin knockdown in MIN6 cells had opposite effects to those observed afte...Continue Reading
References
Sep 1, 1986·Diabetologia·U PantenS Lenzen
Feb 1, 1973·The Biochemical Journal·S J AshcroftP J Randle
Oct 8, 1965·Annals of the New York Academy of Sciences·B Hellman
Jan 15, 1980·The Biochemical Journal·S Lenzen, U Panten
Nov 15, 1993·The Biochemical Journal·M TiedgeS Lenzen
Nov 5, 1997·The Journal of Physiology·L EliassonP Rorsman
Aug 10, 1999·Diabetologia·M TiedgeS Lenzen
Oct 17, 2001·The Journal of Clinical Investigation·G ZhouD E Moller
Dec 28, 2002·Diabetes·Anna Karenina Azevedo-MartinsMarkus Tiedge
Jan 27, 2004·The Journal of Biological Chemistry·Ulrika AnderssonCaroline J Small
Jul 2, 2004·Nature Medicine·Caroline J SmallStephen R Bloom
Mar 19, 2005·The Journal of Biological Chemistry·Makoto TsuneokaYoshiro Koda
Mar 25, 2005·Biochemical Society Transactions·L G D Fryer, D Carling
Oct 4, 2005·The Journal of Clinical Investigation·Isla OgilvieEric A Shoubridge
Nov 19, 2005·The Journal of Endocrinology·S K RichardsR M Smith
Nov 25, 2005·Diabetes·Miriam CnopDecio L Eizirik
Sep 19, 2006·Endocrinology·Simone BaltruschSigurd Lenzen
Apr 6, 2007·Life Sciences·Helena M XimenesSigurd Lenzen
Apr 18, 2007·Molecular and Cellular Biology·Michael LazarouMichael T Ryan
May 17, 2008·Biochemical Society Transactions·Sigurd Lenzen
Jul 10, 2008·Molecular and Cellular Endocrinology·Guy A Rutter, Isabelle Leclerc
Sep 5, 2008·Diabetologia·J XuY Q Liu
Apr 1, 2009·BMC Cell Biology·Paulina WegrzynJolanta Jura
Feb 18, 2010·The Journal of Biological Chemistry·Ewa Gurgul-Convey, Sigurd Lenzen
Feb 25, 2010·Science Signaling·Decio L Eizirik, Miriam Cnop
Oct 26, 2010·Diabetes·Matthias ElsnerSigurd Lenzen
Apr 14, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ewa Gurgul-ConveySigurd Lenzen
May 3, 2011·Cytokine·Stella KachevaEwa Gurgul-Convey
Citations
Jun 3, 2015·Cancer Science·Ming ChenLingya Pan
Jun 2, 2015·Biochemical and Biophysical Research Communications·Ewa Gurgul-ConveySigurd Lenzen
Oct 27, 2017·The Journal of Biological Chemistry·Claudine HahnEwa Gurgul-Convey
Mar 14, 2017·BioMed Research International·Nina Mickelson WeldinghAnette Kocbach Bølling
Jan 12, 2019·Cell Death & Disease·Karolina TykaEwa Gurgul-Convey
Aug 15, 2014·Chemical Communications : Chem Comm·L PasternakA Gruzman
Sep 18, 2014·Experimental Biology and Medicine·Wen KongDi Hu
Aug 8, 2020·Cells·Ewa Gurgul-Convey