Effects of the thermal environment on articular chondrocyte metabolism: a fundamental study to facilitate establishment of an effective thermotherapy for osteoarthritis

Journal of the Japanese Physical Therapy Association = Rigaku Ryōhō
Akira ItoHiroshi Kuroki

Abstract

To facilitate establishment of an effective thermotherapy for osteoarthritis (OA), we investigated the effects of the thermal environment on articular chondrocyte metabolism in vitro. Chondrocytes were isolated from porcine knee joints, and cultured at 32°C, 37°C and 41°C. Cell proliferation and viability were assessed at Days 2, 4 and 8. In addition, TdT-mediated dUTP nick end labeling (TUNEL) assay was performed at Day 3 to determine the proportion of apoptotic chondrocytes. Analysis of genes specific for factors related to the cartilage extracellular matrix (ECM), cartilage destruction, and cartilage protection was performed at Day 2. Furthermore, evaluation of heat stress tolerance, and heat shock protein 70 (HSP70) mRNA expression and protein synthesis was performed at Day 2 and 3, respectively. Cell proliferation was more at 37°C than at 32°C and 41°C. Cell viability and the number of TUNEL-positive cells were not affected until Day 8 and 3, respectively. The expression of the ECM-related genes was up-regulated at higher temperature. The expression of MMP13, a type II collagen destructive enzyme, and that of TIMP1 and TIMP2, which are MMP inhibitors, were up-regulated at higher temperatures. Finally, the chondrocytes cult...Continue Reading

References

May 1, 1977·Radiology·W C DeweyL E Gerweck
May 1, 1989·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M A Mackey, W C Dewey
Mar 1, 1989·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·D N WheatleyD W Gregory
Oct 1, 1994·Seminars in Arthritis and Rheumatism·F G Oosterveld, J J Rasker
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C Shui, A Scutt
Mar 13, 2003·Arthritis and Rheumatism·Wael KafienahMichael D Barker
May 15, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M W DewhirstP J Hoopes
May 28, 2003·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Tatsuya HojoToshikazu Kubo
Jun 10, 2003·Arthritis and Rheumatism·Ryu TerauchiToshikazu Kubo
Oct 30, 2003·The Cochrane Database of Systematic Reviews·L BrosseauP Tugwell
Dec 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne McArdleMalcolm J Jackson
May 14, 2005·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Guillermo Sánchez-InchaustiCarlos Vidal-Fernández
Jun 24, 2006·The Biochemical Journal·Mohamed B Al-Fageeh, C Mark Smales
Sep 1, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hitoshi TonomuraToshikazu Kubo
Oct 10, 2008·The Cochrane Database of Systematic Reviews·Marlene Fransen, Sara McConnell
Dec 10, 2008·Lasers in Surgery and Medicine·Josh T BeckhamE Duco Jansen
Oct 23, 2009·Journal of Drug Targeting·Joseph J MorrisseyPhilip Dixon
May 20, 2011·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Pavel S YarmolenkoMark W Dewhirst
Aug 20, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shinya FujitaToshikazu Kubo
Nov 14, 2013·Journal of Gastroenterology and Hepatology·Libo LiYabing Xin

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