PMID: 16634332Apr 26, 2006Paper

Effects of therapeutic agents on cellular respiration as an indication of metabolic activity

Human & Experimental Toxicology
T IshikawaH Maeda

Abstract

Animal experiments are indispensable in the investigation of the toxicity of drugs on cells, but may not be preferred for ethical reasons and sensitivity. As an alternative procedure, we investigated the susceptibility of cells to drugs using the effect on cellular respiration as an indicator of cell activity (toxicity). The primary cultures (cell lines) used in this study included human fetal myocardial cells, skeletal muscle cells, nerve cells, hypophyseal cells, epithelial cells of gastric mucosa, lymphocytes, hepatocytes, pancreatic (exocrine) cells, renal tubular epithelial cells and fetal adrenal cortex cells, which were obtained from the American Type Culture Collection (ATCC). The drugs used were diazepam, haloperidol and levomepromazine maleate (psychoactive drugs), cisplatin and doxorubicin hydrochloride (anticancer agents). The cells were used at a density of 2 x 10(6) cells/2 mL of growth medium and, to test the susceptibility, each drug was prepared at a concentration of 10 g/mL. Experiment results indicated that, even with the same drug, sensitivity was markedly different depending on the cell lines. Cardiac muscle cells showed the strongest respiratory inhibition by Serenace and were least inhibited by Hirnamin. ...Continue Reading

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Citations

May 9, 2008·International Journal of Clinical Oncology·Seiji IsonishiTadao Tanaka
May 24, 2008·Ecotoxicology and Environmental Safety·Roberto DragoneLuigi Campanella
Jan 24, 2009·British Journal of Cancer·K van NorrenE M van der Beek
Mar 9, 2020·Human Cell·Naoto TaniTakaki Ishikawa
Sep 1, 2010·Experimental and Therapeutic Medicine·Ryuma MatsumotoHiroshi Ishikawa
Nov 24, 2006·Toxicology and Industrial Health·Takaki IshikawaHitoshi Maeda

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