PMID: 7437428Nov 18, 1980Paper

Effects of thiol-reactive agents on amino acid transport by sheep erythrocytes

Biochimica Et Biophysica Acta
J D Young

Abstract

(1) Sheep erythrocytes possess a facilitated diffusion transport system selective for small neutral amino acids of intermediate size (C-system). The effects of seven thiol-reactive agents on this system were investigated. (2) L-Alanine influx by this route was inhibited by HgCl2, p-chloromercuriphenylsulphonate (PCMBS), azodiacarboxylic acid bisdimethylamide (diamide), N-ethylmaleimide and t-butylhydroperoxide. Iodoacetamide and 5,5'-dithiobis(2-nitrobenzoate) had no effect. (3) Detailed analysis of these inhibitor effects suggested the presence of three distinct classes of cellular thiol groups essential for normal transport function. (4) Class 1 thiols react with PCMBS and are located on the outer surface of the cell membrane in the region of the transport site. Class 2 thiols react with N-ethylmaleimide and diamide but are not affected by t-butylhydroperoxide. Class 3 thiols are oxidized during t-butylhydroperoxide treatment and are presumably also attacked by diamide and N-ethylmaleimide. (5) Class 3 thiols are either intracellular GSH or reactive thiols which readily form mixed disulphides with GSSG. Any direct involvement of GSH in amino acid transport is not mediated by the gamma-glutamyl cycle.

References

Jun 28, 1979·Nature·J D YoungJ C Ellory
Aug 1, 1979·Biochemical Society Transactions·J D Young
Jan 15, 1977·The Biochemical Journal·J D Young, J C Ellory
Jan 15, 1976·The Biochemical Journal·J D YoungE M Tucker
Apr 6, 1973·Science·A Meister
Sep 1, 1972·The Journal of Pathology·C L Berry, J Germain
Aug 1, 1973·The Journal of General Physiology·J F SchaefferP F Curran
Jan 1, 1974·Animal Blood Groups and Biochemical Genetics·N S AgarJ D Harley
May 1, 1972·Metabolism: Clinical and Experimental·J D Gardner, A G Levy
Mar 1, 1972·The Journal of Physiology·D G Hoare
Apr 1, 1967·Journal of Cellular Physiology·R M SutherlandR I Weed
Apr 1, 1980·Social Science & Medicine. Medical Anthropology·A Young
May 23, 1980·Biochimica Et Biophysica Acta·R RosenbergJ C Ellory
Sep 1, 1980·American Journal of Diseases of Children·D Young
Mar 1, 1965·The Journal of General Physiology·J VANSTEVENINCKA ROTHSTEIN
Jan 1, 1955·Methods of Biochemical Analysis·J W PATTERSON, A LAZAROW

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Citations

Jan 1, 1981·The Journal of Membrane Biology·H J Sips, K van Dam
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J Lerner, D L Larimore

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