PMID: 9550299Apr 29, 1998Paper

Effects of three peptidase inhibitors, amastatin, captopril and phosphoramidon, on the hydrolysis of [Met5]-enkephalin-Arg6-Phe7 and other opioid peptides

Naunyn-Schmiedeberg's Archives of Pharmacology
T HiranumaT Oka

Abstract

The contents of [Met5]-enkephalin-Arg6-Phe7 (met-enk-RF) and its six hydrolysis products: Y, YG, YGG, YGGF, YGGFM, and YGGFMR were estimated after incubating met-enk-RF with either a guinea-pig ileal or striatal membrane fraction for various times at 37 degrees C. After 45 min incubation with either ileal or striatal membranes, met-enk-RF was completely hydrolyzed, yielding Y as the major product. Incubation with either membrane preparation for 60 min in the presence of the aminopeptidase inhibitor amastatin hydrolyzed 90 or 92% of met-enk-RF, respectively, with YGG being the major product. If the dipeptidyl carboxypeptidase I inhibitor captopril is also included in the incubation, met-enk-RF hydrolysis decreases by about half for both membranes, with YGG remaining the major product. Inclusion of three peptidase inhibitors, amastatin, captopril, and phosphoramidon (inhibition of endopeptidase-24.11) further reduced met-enk-hydrolysis, with 87% or more remaining intact. This shows that met-enk-RF was mainly hydrolyzed by three enzymes, amastatin-sensitive aminopeptidase, captopril-sensitive dipeptidyl carboxypeptidase I and phosphoramidon-sensitive endopeptidase-24.11, in both ileal and striatal membranes. Additionally, estimati...Continue Reading

Citations

Feb 28, 2001·European Journal of Pharmacology·T YamazakiH Ono
Jun 21, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Engin BojnikSandor Benyhe
Nov 16, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Zahra RezaeeSanaz Nasoohi
Feb 26, 2008·Journal of Pharmacological Sciences·Kazuhito AkahoriTetsuo Oka
May 21, 2016·Medicinal Research Reviews·Leonid N MaslovJames M Downey
Jan 27, 1999·Japanese Journal of Pharmacology·T TaniguchiT Oka
Sep 20, 2007·Journal of Pharmacological Sciences·Shigeru TakahashiTetsuo Oka
Feb 28, 2002·The Journal of Pharmacology and Experimental Therapeutics·DeWayne Townsend, David R Brown
Jul 2, 2020·Molecular Pharmacology·Gabrielle C GregoriouElena E Bagley

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