PMID: 9439391Jan 24, 1998Paper

Effects of thrombin and protease nexin-1 on peripheral nerve regeneration

The Annals of Otology, Rhinology, and Laryngology
P LeeD G Roufa

Abstract

Thrombin and serine protease inhibitors such as protease nexin-1 (PN-1) have been implicated in neurite outgrowth activity. We compared rat sciatic nerve regeneration in 10-mm silicone tubes, bridging an 8-mm nerve gap, that were prefilled with thrombin (1.5 IU) and PN-1 (50 micrograms/mL or 1 mg/mL) to those filled with saline solution (control). Neural regeneration and fibrinoid matrix progression (deposition of extracellular matrix) were analyzed at 1, 4, 17, and 21 days after silicone tube implantation. At 1 and 4 days after implantation, thrombin reduced fibrinoid matrix length propagation from both the proximal and distal transected nerve stumps, but PN-1 and saline did not interfere with matrix progression (p < .05). Seventeen days after implantation, the number of silicone tubes containing myelinated neuronal regenerates at the mid-tube region was 1 of 6 for thrombin, 6 of 9 for PN-1, and 6 of 10 for saline solution. Twenty-one days after implantation, 11 of 11 tubes with saline solution, 9 of 11 with PN-1 at 1 mg/mL, and 7 of 9 with PN-1 at 50 micrograms/mL had myelinated neural regenerates in the mid-silicone tube region, while only 2 of 9 thrombin-containing silicone tubes contained myelinated axons. There was no sta...Continue Reading

References

Dec 31, 1992·Annals of the New York Academy of Sciences·D D Cunningham
Jul 1, 1992·The Laryngoscope·J G SpectorD G Roufa
Jan 1, 1990·Journal of Cellular Physiology·D Gurwitz, D D Cunningham
Mar 22, 1988·Biochemistry·W E Van NostrandD D Cunningham
Jul 1, 1993·Archives of Otolaryngology--head & Neck Surgery·D J Terris, W E Fee

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Citations

Nov 21, 2000·Minerva medica·M CamiciR Di Mitri
Jan 1, 1997·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·J G Spector
Sep 6, 2000·Thrombosis Research·V L TurgeonL J Houenou

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