PMID: 9633882Jun 20, 1998Paper

Effects of thrombopoietin on the proliferation and differentiation of primitive and mature haemopoietic progenitor cells in cord blood

British Journal of Haematology
Laurus F SchipperWillem E Fibbe

Abstract

Thrombopoietin (TPO) is considered to be the primary growth factor for regulating megakaryopoiesis and thrombopoiesis. In this study we investigated the in vitro effect of TPO on relatively immature and mature CD34+ progenitor cells in cord blood. Cells were cultured in both liquid and semi-solid cultures containing 50 ng/ml TPO. The CD34+/CD45RA- and CD34+/CD38- subfractions in cord blood were both enriched for megakaryocyte progenitors as determined in a semisolid CFU-meg assay. Progenitor cells derived from the CD34+/CD45RA- and CD34+/CD38- subfractions showed high proliferative capacity in liquid cultures. We observed a mean 19-fold expansion of the total CD34+ cell fraction, whereas in the CD34+/CD45RA- and CD34+/CD38- subfractions the mean expansion was 23- and 50-fold respectively. The expansion of the immature progenitor cell subfractions resulted in a highly purified megakaryocyte suspension containing > 80% megakaryocytes after 14 d in culture. However, these expanded megakaryocytes remained in a diploid (2N) and tetraploid (4N) state. Maturation could not be further induced by low concentration of TPO (0.1 ng/ml). The majority of the cells were 2N (80%) and 4N (15%) and only 5% of the cells had a ploidy of more than ...Continue Reading

References

Jun 1, 1997·British Journal of Haematology·K UsukiA Urabe
Jan 23, 1998·American Journal of Infection Control·S T MerrickJ L Jacobs

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Citations

Apr 15, 2003·Experimental Hematology·Laurus F SchipperWillem E Fibbe
Jan 23, 1999·Current Opinion in Cell Biology·C T Jordan, G Van Zant
Apr 12, 2000·British Journal of Haematology·R MiyazakiY Kobayashi
May 5, 1999·Current Opinion in Hematology·M W Long
Oct 30, 2012·Cellular & Molecular Biology Letters·Viviana M Rodríguez-Pardo, Jean Paul Vernot
Jan 27, 2011·Journal of Biomedical Materials Research. Part a·Vipuil KishoreHoward W T Matthew
Nov 30, 1999·Hematology/oncology Clinics of North America·P J Henslee-Downey, E Gluckman
Jul 19, 2001·Annals of the New York Academy of Sciences·W E FibbeR Willemze

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