Abstract
Cerebrospinal fluid (CSF) is considered highly labile, but not all samples are analyzed immediately. Changes in the composition of CSF could potentially affect diagnostic test results and thus influence decisions about patient management. There has been little scientific inquiry into how variables such as time, initial composition, and storage conditions affect results of standard laboratory analysis of CSF. The objectives of this study were to determine the effects of time, protein concentration, and presence or absence of exogenous stabilizing agents on standard CSF analysis results. Thirty abnormal CSF samples from 26 dogs were evaluated. Samples were divided into aliquots comprising different treatment groups and stored at 4 degrees C. Total nucleated cell count (TNCC), differential cell count (DCC), and cell morphology were evaluated for all groups; protein concentration was measured for selected groups. Unaltered aliquots were analyzed immediately (T0Hr) and at 2, 4, 8, 12, 24, and 48 hours (T2Hr-T48Hr); aliquots with added fetal calf serum (FCS) or hydroxyethyl starch (hetastarch) were analyzed at T48Hr. Significant time-dependent changes were observed in DCC in unaltered samples. Mononuclear cells deteriorated more rapi...Continue Reading
Citations
Mar 15, 2011·Journal of Neurology·Marieke T de GraafPeter A E Sillevis Smitt
Nov 28, 2009·The Veterinary Clinics of North America. Small Animal Practice·William B Thomas
Feb 26, 2008·The Veterinary Journal·Roberta Di Terlizzi, Simon R Platt
Nov 14, 2014·Pathology·Shobini SivagnanamSebastiaan J Van Hal
May 14, 2011·Cytometry. Part B, Clinical Cytometry·Marieke T de GraafJan W Gratama
Mar 17, 2010·Journal of Veterinary Emergency and Critical Care·Peter P Nghiem, Scott J Schatzberg
Aug 14, 2015·BMC Veterinary Research·C BellinoA D'Angelo
Jul 4, 2013·The Veterinary Journal·Intan N F ShafieMark McLaughlin
Aug 16, 2016·The Veterinary Clinics of North America. Small Animal Practice·A Russell Moore
Apr 22, 2016·Equine Veterinary Journal·K J HughesA E Durham
Apr 21, 2016·Journal of Feline Medicine and Surgery·Stefanie GruendlKatrin Hartmann
Mar 12, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Paula M KrimerScott J Schatzberg
Sep 10, 2010·Veterinary Pathology·F Reggeti, D Bienzle
Jun 28, 2018·Veterinary Clinical Pathology·Laura Z HaysomElizabeth A Spangler
May 8, 2019·Acta Veterinaria Scandinavica·Bodil Cathrine KochHanne Gredal
Mar 20, 2020·Veterinary Clinical Pathology·Lindsey N PetersonStephanie A Thomovsky
May 14, 2020·Diagnostic Cytopathology·Kanika RastogiRanjan Gupta
Nov 20, 2016·Veterinary Clinical Pathology·Ricardo MarcosMario Caniatti
Dec 6, 2017·The Journal of Small Animal Practice·P L NewtonM P Best
Feb 24, 2021·The Veterinary Clinics of North America. Equine Practice·Francisco O Conrado, Sarah S K Beatty
Apr 21, 2009·The Veterinary Record·A D'AngeloA Cagnasso
Jan 25, 2011·The Veterinary Record·I MaherK Papasouliotis
Oct 23, 2009·Journal of Proteome Research·Therese RosenlingRainer Bischoff
Aug 26, 2021·The Journal of Small Animal Practice·I Montañes SanchoA Holmes