PMID: 8964760Mar 1, 1996Paper

Effects of tissue fractionation on exercise-induced alterations in SR function in rat gastrocnemius muscle

Journal of Applied Physiology
E R Chin, H J Green

Abstract

Because studies into exercise-induced alterations in sarcoplasmic reticulum (SR) Ca2+ sequestration have produced conflicting reports, we have hypothesized that the differences in SR Ca(2+)-adenosinetriphosphatase (ATPase) activity and Ca2+ uptake in SR fractions observed in different studies are due to different SR isolation techniques. To investigate this possibility, rat white and red gastrocnemius muscles from control and run animals were studied by using two conventional isolation techniques to obtain a crude microsomal fraction and an isolated SR vesicle (SRV) fraction. Indexes of CM and SRV function were compared with measurements from whole muscle homogenate. Treadmill running to exhaustion did not alter SR protein yields, percent SR extraction, or basal or Ca(2+)-ATPase purification in either fraction. Ca(2+)-activated ATPase activity was not altered by exercise in any of the fractions examined, but Ca2+ uptake was reduced in the homogenates (9.48 +/- 1.4 to 6.90 +/- 0.8 nmol . mg-1.min-1) and SRV fractions (84.0 +/- 11.5 to 50.7 +/- 14.0 nmol . mg-1.min-1) from the red gastrocnemius at free Ca2+ concentrations of 600-700 nM. These data indicate that reductions in SR Ca2+ uptake are dissociated from changes in Ca(2+)-A...Continue Reading

Citations

Aug 24, 2000·Journal of Applied Physiology·D Danieli-BettoR Betto
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·J D SchertzerA R Tupling
Oct 17, 2002·Journal of Applied Physiology·J R FowlesA R Tupling
Nov 1, 2002·American Journal of Physiology. Endocrinology and Metabolism·J D SchertzerA R Tupling
Jul 29, 2003·Journal of Applied Physiology·J R FowlesJ Ouyang
May 13, 1998·Acta Physiologica Scandinavica·H J Green
Jun 29, 2004·Journal of Applied Physiology·T A DuhamelJ Ouyang
Jun 17, 2004·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·A Russell Tupling
Oct 24, 2002·Acta Physiologica Scandinavica·S MatsunagaM Wada

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