Effects of titanium-coated micromachined grooved substrata on orienting layers of osteoblast-like cells and collagen fibers in culture

Journal of Biomedical Materials Research. Part a
A KhakbaznejadD M Brunette

Abstract

Osteogenic cells from newborn rat calvariae were cultured on titanium surfaces on which cell orientation could be manipulated. Substrata included smooth surfaces and substrata with smooth regions (gaps) flanked by grooves of 47-microm pitch and 3-, 10-, or 30-microm depth. Orientation angles of the cells were measured over time using propidium-iodide staining and confocal laser scanning microscopy. In addition, collagen fibers were identified using picro-sirius staining and reflected light polarization microscopy. Grooves proved effective in orienting cells, but their orienting ability decreased above the ridge level. Cells on the smooth surface showed no preferred orientation. Cells in the gaps became oriented as a result of cell-cell interactions with the cells on the flanking grooves. Cells in grooves produced oriented collagen fibers, but in the gaps, fibers could be parallel, perpendicular, or diagonal to the grooves. Collagen fibers on the smooth surfaces formed arrays of parallel fibers in a crisscross pattern. In long-term cultures, bone-like nodules were formed, but mostly above the ridge level. These data demonstrate that grooved surfaces can influence cell orientation both in cell populations above the cells in conta...Continue Reading

References

Jan 1, 1977·Acta Anatomica·P FrascaJ L Katz
May 27, 1975·Cell and Tissue Research·S J JonesJ B Pawley
May 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Ben-Ze'ev
Jan 1, 1990·Calcified Tissue International·M Casser-BetteJ Schmidt
May 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D E IngberJ Folkman
Jan 1, 1989·Matrix : Collagen and Related Research·R J KlebeS Milam
Mar 1, 1986·Calcified Tissue International·C G BellowsM E Antosz
Sep 1, 1987·Journal of Electron Microscopy Technique·U Aebi, T D Pollard
Nov 1, 1986·Experimental Cell Research·D M Brunette
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Jun 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R L Trelstad
Aug 1, 1967·The Anatomical Record·A Ascenzi, E Bonucci
Jul 1, 1968·The Anatomical Record·A Ascenzi, E Bonucci
May 25, 1999·Periodontology 2000·R K Schenk, D Buser
Jun 24, 1999·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Jul 6, 2000·Journal of Biomedical Materials Research·X F WalboomersJ A Jansen
Jul 13, 2002·Journal of Biomedical Materials Research·J Glass-BrudzinskiD M Brunette

❮ Previous
Next ❯

Citations

Jul 5, 2007·Journal of the Royal Society, Interface·Günter WrobelAndreas Offenhäusser
Nov 10, 2009·Cell and Tissue Research·Klaus von der MarkPatrik Schmuki
Mar 15, 2005·Connective Tissue Research·Solfrid ArnesenMoira Lawson
Nov 9, 2012·Journal of Clinical Periodontology·Victoria R KearnsNicolas Martin
Oct 2, 2007·Journal of Biomedical Materials Research. Part a·H KenarV Hasirci

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.