Effects of titanium dioxide nanoparticle aggregate size on gene expression.

International Journal of Molecular Sciences
Junko Okuda-ShimazakiAkiyohshi Taniguchi

Abstract

Titanium dioxide (titania) nanoparticle aggregation is an important factor in understanding cytotoxicity. However, the effect of the aggregate size of nanoparticles on cells is unclear. We prepared two sizes of titania aggregate particles and investigated their biological activity by analyzing biomarker expression based on mRNA expression analysis. The aggregate particle sizes of small and large aggregated titania were 166 nm (PDI = 0.291) and 596 nm (PDI = 0.417), respectively. These two size groups were separated by centrifugation from the same initial nanoparticle sample. We analyzed the gene expression of biomarkers focused on stress, inflammation, and cytotoxicity. Large titania aggregates show a larger effect on cell viability and gene expression when compared with the small aggregates. This suggests that particle aggregate size is related to cellular effects.

Associated Clinical Trials

References

Jun 1, 1992·Immunological Reviews·S Akira, T Kishimoto
Dec 5, 1998·Pharmacology & Therapeutics·J G Kiang, G C Tsokos
Nov 16, 2001·Seminars in Immunology·P Sansonetti
Nov 1, 1995·Trends in Cell Biology·J A Swanson, C Watts
Jun 19, 2004·Science·Robert F Service
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Feb 4, 2006·Science·Andre NelNing Li
Feb 24, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·David B WarheitKenneth L Reed
Oct 13, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·David B WarheitChristie M Sayes
Feb 28, 2008·The Journal of Toxicological Sciences·Atsuya TakagiJun Kanno
Apr 17, 2008·Environmental Health Perspectives·Philippe Baveye, Magdeline Laba
Aug 2, 2008·The Journal of Toxicological Sciences·Gaku IchiharaKun'ichi Miyazawa
Dec 17, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katrina M WatersBrian D Thrall
May 19, 2009·Toxicology Letters·Hanna L KarlssonLennart Möller
May 22, 2009·Bio-medical Materials and Engineering·Masayuki TairaYoshima Araki

❮ Previous
Next ❯

Citations

Dec 6, 2011·Chemical Research in Toxicology·Masanori HorieHitoshi Iwahashi
Nov 13, 2012·International Journal of Nanomedicine·Eleonore Fröhlich
Dec 19, 2013·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Angelica SetteAnnarosa Arcangeli
Dec 23, 2015·Nanotoxicology·Jose E PerezJürgen Kosel
Mar 8, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Michele MunkHumberto M Brandão
Nov 22, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Neus FeliuWolfgang J Parak
Dec 15, 2012·Toxicology·Andrea StoccoroLucia Migliore
Dec 29, 2016·Journal of Biomedical Materials Research. Part a·Elanur AydınFatime Geyikoğlu
Apr 11, 2012·International Journal of Molecular Sciences·Sharmy Saimon ManoAkiyoshi Taniguchi
Jun 28, 2013·International Journal of Molecular Sciences·Sharmy Saimon ManoAkiyoshi Taniguchi
Oct 12, 2018·Clinical Oral Implants Research·Andrea MombelliNorbert Cionca
Oct 3, 2019·Brazilian Oral Research·Daniela Leal Zandim-BarcelosGiuseppe Alexandre Romito
Jul 10, 2019·International Journal of Implant Dentistry·Frank SchwarzJürgen Becker
Mar 7, 2013·Science and Technology of Advanced Materials·Peng ChenAkiyoshi Taniguchi
Nov 24, 2016·Science and Technology of Advanced Materials·Qingqing SunAkiyoshi Taniguchi
Feb 14, 2018·Nanoscale·Stefán B GunnarssonTommy Cedervall
Aug 3, 2017·Journal of Periodontal Research·M Noronha OliveiraJ C M Souza
Feb 1, 2017·Journal of Toxicology and Environmental Health. Part a·Sreeparna ChakrabortyGiovanni Piedimonte
Oct 29, 2020·Journal of Applied Toxicology : JAT·Eşref Demir
Jun 17, 2021·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Tyler NguyenXiaoming Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
dynamic light scattering
Assay
mechanical dissociation
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.