Effects of TONS504-photodynamic therapy on mouse mammary tumor cells

Oncology Letters
Tomohiro OsakiYoshiharu Okamoto

Abstract

In the present study, TONS504 (C51H58N8O5I2; molecular weight, 1,116.9), a novel cationic hydrophilic photosensitizer, was synthesized from protoporphyrin IX dimethyl ester through a five-step process according to a patented method for use in photodynamic therapy (PDT). The subcellular localization of TONS504 and the cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated. TONS504 was localized primarily in the lysosomes and partially in the mitochondria. The cytotoxic effects of TONS504-mediated PDT in the mouse mammary tumor EMT6 cell line were investigated using a WST8 assay and an Oxidative Stress kit. The cell viability values following treatment with 10 µg/ml TONS504 at light energies of 0, 1, 5 and 10 J/cm2 were 92.5, 101.8, 27.7 and 1.8%, respectively. The percentages of reactive oxygen species (ROS)(+) cells following the same treatment were 8.6, 8.5, 29.2 and 70.1%, respectively, whereas the percentages of apoptotic cells were 7.1, 5.6, 24.8 and 48.7%, respectively. The percentages of ROS(+) and apoptotic cells in the group subjected to TONS504-mediated PDT increased in a manner dependent on the TONS504 concentration and light energy. Further studies are required to evalua...Continue Reading

References

Oct 13, 2006·The Oncologist·Martijn TriesscheijnFiona A Stewart
Jul 6, 2010·Journal of Photochemistry and Photobiology. B, Biology·Juergen BerlandaKristjan Plaetzer
Oct 31, 2014·Lasers in Medical Science·Miftahul Akhyar LatiefAkira Obana
Sep 4, 2015·International Journal of Molecular Sciences·Isabel O L BacellarMauricio S Baptista
Oct 27, 2015·Anti-cancer Agents in Medicinal Chemistry·Blassan Plackal Adimuriyil George, Heidi Abrahamse
Nov 12, 2016·The Breast : Official Journal of the European Society of Mastology·S M BanerjeeM R S Keshtgar
Apr 5, 2017·Anticancer Research·Tomohiro OsakiYoshiharu Okamoto
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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Methods Mentioned

BETA
fluorescence microscopy
Assay

Software Mentioned

GraphPad Prism
GraphPad
IncuCyte

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