Effects of topical application of methotrexate on nasal mucosa in rats: a preclinical assessment study

Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery
Ibrahim ErcanSuat Turgut

Abstract

The aim of this study is to investigate dose-dependent effect of the topical application of methotrexate (MTX) in rats on the normal nasal mucosa, liver tissue, liver enzymes, and hemoglobin levels. Preclinical animal study. Twenty male adult wistar albino rats were randomly divided into 4 groups (n=5). A single puff of MTX (2.5 microg) was applied to both nasal cavities 2 times a day. The animals were given MTX 1 day a week in group 1, 3 days a week in group 2, and 5 days a week in group 3. Control group animals were given 1 puff of physiologic saline to both nasal cavities 5 days a week and 2 times a day. After 28 days, liver biopsies, blood samples, and 5 nasal mucosal biopsies were taken. Histological examination was made with respect to certain parameters semiquantitatively (grade 0-3). The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and hemoglobin counts were studied from blood samples. There are no statistically significant differences with respect to histopathological parameters between the control group and the groups 1-3 (P>0.05). Histopathological examination of liver tissue did not reveal any evident difference between the control and study groups. Mean AST and ALT as liver function te...Continue Reading

References

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Citations

Dec 12, 2012·The Journal of Laryngology and Otology·E SozenB Dadas
Mar 13, 2016·International Journal of Pediatric Otorhinolaryngology·Murat Salih GüneşBarış Derya Erçal
Jun 12, 2014·Clinical and Experimental Otorhinolaryngology·Mehmet Ozgür AvincsalEnis Alpin Güneri
May 12, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Mustafa SagitMehmet Yasar
Oct 27, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Erol SenturkIlker Kocak

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Methods Mentioned

BETA
biopsies
biopsy

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