Abstract
The aim of this study is to investigate dose-dependent effect of the topical application of methotrexate (MTX) in rats on the normal nasal mucosa, liver tissue, liver enzymes, and hemoglobin levels. Preclinical animal study. Twenty male adult wistar albino rats were randomly divided into 4 groups (n=5). A single puff of MTX (2.5 microg) was applied to both nasal cavities 2 times a day. The animals were given MTX 1 day a week in group 1, 3 days a week in group 2, and 5 days a week in group 3. Control group animals were given 1 puff of physiologic saline to both nasal cavities 5 days a week and 2 times a day. After 28 days, liver biopsies, blood samples, and 5 nasal mucosal biopsies were taken. Histological examination was made with respect to certain parameters semiquantitatively (grade 0-3). The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and hemoglobin counts were studied from blood samples. There are no statistically significant differences with respect to histopathological parameters between the control group and the groups 1-3 (P>0.05). Histopathological examination of liver tissue did not reveal any evident difference between the control and study groups. Mean AST and ALT as liver function te...Continue Reading
References
Feb 1, 1989·Archives of Dermatology·G D WeinsteinE Olsen
Mar 28, 1985·The New England Journal of Medicine·M E WeinblattD E Trentham
May 1, 1985·Clinical and Experimental Dermatology·D A FentonM M Black
Jan 1, 1980·Archives of Oto-rhino-laryngology·C BaumgartenH Gelderblom
Oct 1, 1994·American Journal of Respiratory and Critical Care Medicine·N KanaiJ Dolovich
May 1, 1994·Immunopharmacology·J SchmidtI Szelenyi
Jan 1, 1996·Stem Cells·E ChuC J Allegra
Jun 1, 1997·Archives of Otolaryngology--head & Neck Surgery·T LildholdtK Larsen
Jul 1, 1997·Biopharmaceutics & Drug Disposition·G LuH Suh
Jul 1, 1997·Chest·M G Marin
Jan 20, 1999·Respiratory Medicine·S D AaronB Pham
Apr 12, 2000·The European Respiratory Journal·B VrugtR Aalbers
May 16, 2000·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·I AhmadR T Shortridge
Jun 30, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·R Y LinH Menikoff
Dec 21, 2000·Current Rheumatology Reports·J T Roland
May 6, 2004·The Laryngoscope·Rhoda Wynn, Gady Har-El
Citations
Dec 12, 2012·The Journal of Laryngology and Otology·E SozenB Dadas
Mar 13, 2016·International Journal of Pediatric Otorhinolaryngology·Murat Salih GüneşBarış Derya Erçal
Jun 12, 2014·Clinical and Experimental Otorhinolaryngology·Mehmet Ozgür AvincsalEnis Alpin Güneri
May 12, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Mustafa SagitMehmet Yasar
Oct 27, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Erol SenturkIlker Kocak