PMID: 7524206Oct 15, 1994Paper

Effects of transfected complement regulatory proteins, MCP, DAF, and MCP/DAE hybrid, on complement-mediated swine endothelial cell lysis

Transplantation
S MiyagawaS Nagasawa

Abstract

We established several swine endothelial cell (SEC) lines, expressing human MCP (CD46), DAF (CD55), and MCP/DAF hybrid by transfection of cDNA, and assessed the function of these transfectant molecules on complement-mediated cell lysis as an in vitro hyperacute rejection model of swine to human discordant xenograft. Discordant organ xenografts are hyperacutely rejected by complement activation. Amelioration of complement-mediated lysis by these transfectant molecules was tested in each SEC line by lactate dehydrogenase assay. Naive swine endothelial cells were markedly damaged by human complement mainly via the classical pathway, activating only minimally the alternative pathway of human complement. Both MCP and DAF protected SEC from human complement attack in parallel with the expression density, with DAF being more effective than MCP. The MCP/DAF hybrid was more effective than MCP alone, and as effective as DAF in this system. The results suggest that the transfection of DAF or the MCP/DAF hybrid cDNA into organs to be transplanted could protect against hyperacute rejection.

Citations

Feb 9, 2002·Molecular Reproduction and Development·Hiroshi MurakamiTaroh Kinoshita
Nov 24, 1999·Biochemical and Biophysical Research Communications·M YoshitatsuR Shirakura
Aug 7, 2012·Surgery Today·Aki YamamotoShuji Miyagawa
Aug 11, 2000·Transplantation Proceedings·M KomaN Taniguchi
Aug 11, 2000·Transplantation Proceedings·M YoshitatsuR Shirakura
Aug 11, 2000·Transplantation Proceedings·K MatsunamiR Shirakura
Dec 20, 2000·Transplantation Proceedings·K MatsunamiR Shirakura
Dec 20, 2000·Transplantation Proceedings·M KomaN Taniguchi
Feb 20, 2003·Transplantation Proceedings·K MatsunamiR Shirakura
Feb 20, 2003·Transplantation Proceedings·D FukutaR Shirakura
Feb 20, 2003·Transplantation Proceedings·T KusamaR Shirakura
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ShimizuY Tsujimoto
Oct 27, 2001·Clinical and Experimental Immunology·K MatsunamiR Shirakura
Jan 24, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M TanemuraT Ito
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·H NagashimaM Nagaya
Feb 13, 2010·Xenotransplantation·Shuji MiyagawaMasahiro Fukuzawa
Nov 17, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Aki YamamotoShuji Miyagawa
Mar 28, 2006·Biochemical and Biophysical Research Communications·Katsuyoshi MatsunamiRyota Shirakura
Dec 19, 2006·Transplantation Proceedings·C KoboyashiS Miyagawa
Apr 22, 2004·Xenotransplantation·Hiroshi KomodaRyota Shirakura

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