PMID: 9444946Jan 28, 1998Paper

Effects of transfection of p210bcr-abl and bcr-v-abl into the factor-dependent human leukemia cell line HSM-911

Leukemia Research
M TakahashiY Aizawa

Abstract

In order to clarify the action of the bcr-abl, a growth factor dependent human leukemic cell line (HSM-911) was transfected with p210bcr-abl or bcr-v-abl by electroporation. The cells transfected with bcr-v-abl, but not the cells transfected with p210bcr-abl, became growth factor independent. Some clones of the cells transfected with p210bcr-abl demonstrated cellular maturation (nuclear segmentation, becoming positive for naphthol ASD chloroacetate esterase, the disappearance of CD34 expression and the appearance of glycophorin A and CD10 expression). Moreover, these clones transfected with p210bcr-abl demonstrated apoptosis (increased expression of Fas and DNA ladder formation suggesting apoptotic DNA fragmentation). These findings demonstrated the different actions of p210 bcr-abl and bcr-v-abl, the former of which gave the cells the characteristics of maturation like the cells from chronic myelogenous leukemia, and the latter of which rendered the cells grow autonomously.

References

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G Q Daley, D Baltimore
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J McLaughlinO N Witte
Sep 22, 1995·The Journal of Biological Chemistry·A J McGahonD R Green

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