PMID: 9166720Jun 1, 1997Paper

Effects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors: insights gained from a neuronal cell line

Journal of Neurochemistry
K AsakuraM Rodriguez

Abstract

Conditioned medium derived from a rat central nervous system neuronal cell line B104 (B104 CM) was shown previously to contain uncharacterized potent mitogen(s) for oligodendrocyte/type-2 astrocyte (O-2A) progenitor cells. In this study, we demonstrated that B104 cells produce and secrete platelet-derived growth factor (PDGF)-AA homodimer, but not PDGF-B chain. B104 cells did not express other known potent mitogens for O-2A progenitor cells, including fibroblast growth factor-2 and neurotrophin-3. Unexpectedly, B104 cells also expressed transcripts of transforming growth factor-beta1 (TGF-beta1) and -beta2 (TGF-beta2), which are known to regulate O-2A progenitor cell differentiation and proliferation, and secreted exclusively the 25-kDa active forms of TGF-beta1 and TGF-beta2. Neutralization of B104 CM with anti-PDGF-AA antibody decreased proliferation of O-2A progenitor cells, whereas neutralization with anti-TGF-beta antibodies had no effect. The combination of PDGF and TGF-beta on proliferation was not equivalent to the effect of B104 CM, indicating the possibility of an unidentified growth factor. B104 CM maintained a high expression of PDGF-alpha receptor in oligodendrocytes. The observation that both a stimulatory factor ...Continue Reading

Citations

Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·A E WarringtonM Rodriguez
Nov 7, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cleber A TrujilloHenning Ulrich
Apr 27, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F C GomesV Moura Neto
Nov 13, 2009·Journal of Neuroscience Research·Makoto HoriuchiTakayuki Itoh
Aug 28, 2012·International Journal of Molecular Medicine·Jian-Guo HuHe-Zuo Lü
Sep 11, 2013·Journal of Molecular Neuroscience : MN·Elmira Mikaeili AgahMasoud Soleimani
May 28, 2009·Journal of Neuroscience Research·Sinead M Gibney, Kieran W McDermott
Jul 14, 2006·Neuroreport·Samaneh MaysamiMartin Stangel
Mar 8, 2006·The European Journal of Neuroscience·P M GaughwinS Chandran
Apr 28, 2010·In Vitro Cellular & Developmental Biology. Animal·Yong-Chun LuoRu-Xiang Xu
Aug 30, 2008·Current Protocols in Stem Cell Biology·Eric C LarsenIan D Duncan
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yoshihiro MitsunagaLarry R Pease
Nov 22, 2000·Molecular and Cellular Neurosciences·L DeckerA Baron-Van Evercooren
Aug 5, 2006·Neurobiology of Disease·Charles L HoweMoses Rodriguez
Jun 16, 2010·Experimental Neurology·Amanda L LindkeMichael W Miller

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