Effects of Transgenic Maize with cry1Ab and Epsps Genes C0030.3.5 on the Abundance and Community Structure of Soil Nitrogen-fixing Bacteria

Huan jing ke xue= Huanjing kexue
Rui WangDian-Lin Yang

Abstract

In order to evaluate the potential risk of planting transgenic corn on soil nitrogen-fixing microorganisms, in 2015, rhizosphere and non-rhizosphere soil samples were collected at the jointing stage, tassel stage, milky stage, and ripening stage, and the effects of transgenic maize with the cry1Ab and epsps genes on the abundance and diversity of soil nitrogen-fixing bacteria were studied by real-time quantitative PCR and T-RFLP. The results showed that the copy number of the diazotrophic nifH gene in the rhizosphere and non-rhizosphere soil of transgenic maize with the cry1Ab and epsps genes (C0030.3.5) and its parental maize (DBN318) showed a trend where it first increased and then decrease with the growth stages, ranging between 2.99×107 and 7.02×107 copies·g-1. The abundance of the diazotrophic nifH gene in the rhizosphere soil and non-rhizosphere soil gene showed no significant difference between TM and PM in the same growth stage (P>0.05). The correlation analysis showed that the abundance of the diazotrophic nifH gene was positively correlated with the content of organic matter, but negatively correlated with water content. T-RFLP analysis yielded 14 T-RFs of different lengths, and 43-bp and 155-bp fragments were the dom...Continue Reading

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