Effects of transient coronary occlusion on the capillary network in the left ventricle of rat

The Japanese Journal of Physiology
Z XieT Koyama

Abstract

The objective was to examine the changes in the capillary network in the left ventricle of rats subjected to transient occlusion of the left coronary artery followed by reperfusion (I-R). Eighteen Wistar rats were divided into three groups and all rats were anaesthetized with ethyl ether and artificially ventilated. The I-R 1 rats were subjected to a 3 min occlusion followed by reperfusion; the I-R 3 rats had three 3 min occlusions separated by 3 min of reperfusion; the Sham-operated rats underwent surgery but the coronary artery was not occluded. The thorax was closed at the end of the procedures and the rats were sacrificed for isolation of the hearts 30 d after treatment. Frozen sections of the left ventricles were cut and differential staining was used to classify the capillary portions. Five additional rats treated as the I-R 1 group were sacrificed at 120 min after reperfusion. Their left ventricles were used for immunohistochemical investigation of the early expression of bFGF and VEGF. By comparison with the Sham-operated rats, both I-R groups showed increases in the capillary density of total and venular capillary portions, an increased capillary : myocyte (C : M) ratio and a decrease in the capillary domain area in th...Continue Reading

Citations

Oct 3, 1998·The Japanese Journal of Physiology·T KoyamaS Batra
May 2, 2000·Radiation Research·M GaoT Koyama
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H EndohE Takahashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.