Effects of Transport and Storage Conditions on Gene Expression in Blood Samples

Biopreservation and Biobanking
F MalentacchiS Gelmini

Abstract

Inappropriate handling of blood samples might induce or repress gene expression and/or lead to RNA degradation affecting downstream analysis. In particular, sample transport is a critical step for biobanking or multicenter studies because of uncontrolled variables (i.e., unstable temperature). We report the results of a pilot study implemented within the EC funded SPIDIA project, aimed to investigate the role of transport and storage of blood samples containing and not containing an RNA stabilizer. Blood was collected from a single donor both in EDTA and in PAXgene Blood RNA tubes. Half of the samples were sent to a second laboratory both at room temperature and at 4°C, whereas the remaining samples were stored at room temperature and at 4°C. Gene expression of selected genes (c-FOS, IL-1β, IL-8, and GAPDH) known to be induced or repressed by ex vivo blood handling and of blood-mRNA quality biomarkers identified and validated within the SPIDIA project, which allow for monitoring changes in unstabilized blood samples after collection and during transport and storage, were analyzed by RT-qPCR. If the shipment of blood in tubes not containing RNA stabilizer is not performed under a stable condition, gene profile studies can be aff...Continue Reading

References

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Nov 1, 2012·Methods : a Companion to Methods in Enzymology·M PazzagliS Gelmini

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Citations

May 22, 2019·Clinical Chemistry and Laboratory Medicine : CCLM·Chiara StellinoFay Betsou
Apr 8, 2021·International Journal of Radiation Biology·Farah NasserChristophe Badie

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Methods Mentioned

BETA
blood collection
chips
PCR
Assay
blood
blood drawing

Software Mentioned

SPIDIA

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