Effects of Treated versus Untreated Polystyrene on Caspofungin In Vitro Activity against Candida Species

Journal of Clinical Microbiology
A FothergillNathan P Wiederhold

Abstract

Significant interlaboratory variability is observed in testing the caspofungin susceptibility of Candida species by both the CLSI and EUCAST broth microdilution methodologies. We evaluated the influence of treated versus untreated polystyrene microtiter trays on caspofungin MICs using 209 isolates of four Candida species, including 16 C. albicans and 11 C. glabrata isolates with defined FKS mutations. Caspofungin MICs were also determined using the commercially available YeastOne and Etest assays and 102 isolates. All C. glabrata isolates had caspofungin MICs of ≥0.5 μg/ml, the clinical breakpoint for caspofungin resistance in this species, measured using trays made of treated polystyrene, regardless of the FKS status. In contrast, susceptible isolates could readily be distinguished from resistant/non-wild-type isolates when caspofungin MICs were measured using untreated polystyrene trays and both the YeastOne and Etest assays. Similar results were also observed for C. krusei isolates, as all isolates had caspofungin MICs above the threshold for resistance measured using treated polystyrene trays. In contrast, C. albicans isolates could be correctly identified as susceptible or resistant when caspofungin MICs were measured with...Continue Reading

References

Apr 2, 2008·Antimicrobial Agents and Chemotherapy·John D ClearyDavid S Perlin
Sep 17, 2008·Antimicrobial Agents and Chemotherapy·Nathan P WiederholdSamuel A Lee
Oct 30, 2012·Diagnostic Microbiology and Infectious Disease·Helio S SaderRonald N Jones
Mar 15, 2013·Journal of Clinical Microbiology·Janet A Hindler, Romney M Humphries
Jan 1, 2014·The Journal of Antimicrobial Chemotherapy·Mahableshwar AlburAlasdair Macgowan
Oct 21, 2014·Pharmacotherapy·Romney M Humphries

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Citations

Oct 25, 2016·Current Infectious Disease Reports·Nathan P Wiederhold
May 23, 2018·Antimicrobial Agents and Chemotherapy·Karin Meinike JørgensenMaiken Cavling Arendrup
Jul 10, 2019·Antimicrobial Agents and Chemotherapy·Maiken Cavling ArendrupOscar Zaragoza
Sep 21, 2019·The Journal of Antimicrobial Chemotherapy·Zoltán TóthLászló Majoros
Aug 1, 2020·Frontiers in Cellular and Infection Microbiology·Derry K MercerAlfredo M Angeles-Boza
Mar 19, 2021·The Journal of Antimicrobial Chemotherapy·Maiken Cavling ArendrupPaul E Verweij

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