Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats

Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
Xue-Mei LiuHong Zheng

Abstract

To observe the effects of Tribulus terrestris L. saponion (TTLS) on apoptosis in cortical neurons induced by hypoxia-reoxygenation in rats. Primary culture of rat cortical neurons was performed in vitro. A model of apoptosis of cortical neurons was established by hypoxia and reoxygenation. Hypoxia for 3 h in neural cells was induced with mixture of 95% N(2) and 5% CO(2), and then reoxygenation in neural cells was induced with mixture of 95% O(2) and 5% CO(2) for 12 h. Different concentrations of TTLS were administered to traditional Chinese herbal medicine-treated group separately during hypoxia and reoxygenation. The apoptosis rate was analyzed quantitatively by flow cytometry with Annexin V-FITC and propidium iodide staining. Mitochondria membrane potential was observed by a confocal laser-scanning microscope with JC-1 fluorescence. Caspase-3/7 activity in cytoplasm was measured by fluorescent plate reader. Bax protein expression was observed by immunohistochemical technique. The percentage of apoptosis was significantly increased, mitochondria membrane potential was obviously decreased, fluorescence of caspase-3/7 activity was increased, and Bax protein was abundantly expressed followed by 3 h of hypoxia and 12 h of reoxygen...Continue Reading

References

Aug 26, 1998·Biochimica Et Biophysica Acta·S A SusinG Kroemer
Jan 23, 1999·Oncogene·P SaikumarM A Venkatachalam
Feb 15, 2001·Cell Death and Differentiation·M H Harris, C B Thompson
Nov 28, 2002·Molecular Cell·J Marie Hardwick, Brian M Polster
Mar 27, 2003·Progress in Neuro-psychopharmacology & Biological Psychiatry·Paula C Ashe, Mark D Berry
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·J D LyA Lawen

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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