PMID: 7037058Feb 10, 1982Paper

Effects of trypsin on binding of insulin and concanavalin A and on glucose and proline transport in the R3230AC mammary adenocarcinoma

Biochimica Et Biophysica Acta
R Hilf, D L Helton

Abstract

Effects of trypsin treatment on insulin and concanavalin A binding to, and glucose and proline transport in, dissociated R3230AC mammary adenocarcinoma cells were examined. Reduction of binding of 125I-labelled insulin was dependent on the amount of trypsin used, the temperature and the time of the incubation period. Under conditions that reduced insulin binding by greater than 75%, transport of glucose and proline was reduced by less than 15%. Scatchard analysis of insulin binding after trypsin treatment yielded slopes similar to those from cells not exposed to trypsin, assuming either two classes of receptors or an average affinity, Ke. Dissociation of bound insulin from untreated or trypsin-treated cells was enhanced by addition of excess unlabelled ligand. Insulin added in vitro, which decreased glucose transport in untreated cells, produced a decrease in glucose transport in cells treated with trypsin for 5 min (insulin binding was decreased 35%), but not in cells treated for 45 min (insulin binding was decreased 90%). Binding of the plant lectin concanavalin A was also reduced by trypsin treatment, but to a lesser extent and with a different time-course than for insulin. Scatchard analysis of the binding of concanavalin A...Continue Reading

References

Jan 1, 1976·Journal of Supramolecular Structure·J T Harmon, R Hilf
Apr 4, 1978·Biochimica Et Biophysica Acta·P J Hissin, R Hilf
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P Cuatrecasas, G P Tell
Jun 26, 1972·Biochimica Et Biophysica Acta·R M el-Allawy, J Gliemann
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·P Cuatrecasas
Apr 16, 1971·Biochemical and Biophysical Research Communications·P FreychetD M Neville
Jan 30, 1981·Biochemical and Biophysical Research Communications·H M KatzenB G Green
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lili YangLuk Van Parijs
Jul 5, 1958·British Medical Journal·J M SALTERC H BEST
Mar 16, 2005·Journal of Virology·Byeongwoon SongJoseph Sodroski

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Citations

Jul 18, 1984·Biochemical and Biophysical Research Communications·K Sorimachi, Y Yasumura
Oct 15, 1983·Experientia·A J CrossJ A Johnson

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