Effects of TT8 and HB12 Silencing on the Relations between the Molecular Structures of Alfalfa ( Medicago sativa) Plants and Their Nutritional Profiles and In Vitro Gas Production

Journal of Agricultural and Food Chemistry
Yaogeng LeiPeiqiang Yu

Abstract

The objective of this study was to investigate the effects of silencing the TT8 and HB12 genes on the nutritive profiles and in vitro gas production of alfalfa in relation to the spectral molecular structures of alfalfa. TT8-silenced (TT8i, n = 5) and HB12-silenced (HB12i, n = 11) alfalfa were generated by RNA interference (RNAi) and grown with nontransgenic wild type controls (WT, n = 4) in a greenhouse. Alfalfa plants were harvested at early-to-mid vegetative stage. Samples were analyzed for their chemical compositions, CNCPS fractions, and in vitro gas production. Correlations and regressions of the nutritional profiles and in vitro gas production with the molecular spectral structures were also determined. The results showed that the transformed alfalfa had higher digestible fiber and lower crude protein with higher proportions of indigestible protein than WT. HB12 RNAi had lower gas production compared with those of the others. Some chemical, CNCPS, and gas-production profiles were closely correlated with spectral structures and could be well predicted from spectral parameters. In conclusion, the RNAi silencing of TT8 and HB12 in alfalfa altered the chemical, CNCPS and gas-production profiles of alfalfa, and such alteratio...Continue Reading

References

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Jul 6, 2014·Nature Protocols·Matthew J BakerFrancis L Martin
Dec 24, 2014·Plant Biotechnology Journal·Banyar AungAbdelali Hannoufa
Jan 13, 2015·Trends in Plant Science·Wenjia XuLoïc Lepiniec
Feb 2, 2017·International Journal of Molecular Sciences·Yaogeng LeiPeiqiang Yu
Mar 24, 2017·Plant Science : an International Journal of Experimental Plant Biology·Muhammad ArshadAbdelali Hannoufa

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