PMID: 7627812Apr 1, 1995Paper

Effects of tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6 on type I iodothyronine 5'-deiodination in rat thyroid cell line, FRTL-5

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
H HashimotoT Sato

Abstract

Using a functioning rat thyroid cell line (FRTL-5), we studied the effects of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) on thyroidal type I iodothyronine 5'-deiodination (I-5'-deiodination) and on the expression of I-5'-deiodinase (I-5'-D) mRNA. After 24 h incubation in medium containing 0.5 microM rT3 with a tracer amount of [125I]rT3, radioactivity of released 125I- was counted. Deiodination in live FRTL-5 cells was enhanced about three times from the basal level by the addition of TSH and was inhibited markedly by propylthiouracil and dose dependently by T4. These results suggest the suitability of this model for investigating I-5'-deiodination in live thyroid tissue. Basal and TSH-induced I-5'-deiodination were significantly inhibited by 100 ng/liter of IL-1 beta and IL-6, and the inhibitory effect of TNF-alpha was seen over 1 microgram/liter. I-5'-deiodination was restored by removal of the cytokines. TSH-induced cAMP production and (Bu)2cAMP-induced I-5'-deiodination were also inhibited by the cytokines. Catalase, dexamethasone, and indomethacin did not abolish the inhibitory effects of the cytokines. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed ...Continue Reading

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Citations

Sep 12, 2012·International Urology and Nephrology·Usama ElewaAlberto Ortiz
Mar 4, 2000·Domestic Animal Endocrinology·S KahlJ W Blum
Jun 1, 2001·Journal of Endocrinological Investigation·J PachuckiA Nauman
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May 15, 2015·The Journal of Endocrinology·Emmely M de VriesAnita Boelen
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Aug 30, 2013·The Tohoku Journal of Experimental Medicine·Mao-Hui FengDong-Liang Hu

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