Effects of tyrosine kinase inhibitor on the motility and ATP concentrations of fowl spermatozoa

Molecular Reproduction and Development
K AshizawaYasuhiro Tsuzuki


The possible role of tyrosine kinase in the regulation of fowl sperm motility was investigated by using a stable analogue of erbstatin, methyl 2,5-dihydroxycinnamate (2,5-MeC), a specific inhibitor of tyrosine kinase. This inhibited the motility of intact spermatozoa at 30 degrees C in a dose-dependent manner. In contrast, the motility of demembranated spermatozoa was not inhibited by the same concentrations of 2,5-MeC. At 40 degrees C, both intact and demembranated spermatozoa were almost immotile with or without 2,5-MeC. Additionally, intact spermatozoa, stimulated by the addition of Ca2+ or calyculin A, a specific inhibitor of protein phosphatases, lost their motility with the subsequent addition of 2,5-MeC at 40 degrees C. However, unlike the motility, the ATP concentrations of spermatozoa were maintained in about 30-35 nmol ATP/10(9) cells during these incubation periods. The activity of tyrosine kinase of spermatozoa at 30 degrees C, estimated by measuring the phosphorylation of a synthetic peptide substrate, RR-SRC, was 0.17 pmol/min per milligram of protein. This activity was lower than that of fowl testes or chick brain but higher than that of chick liver. These results suggest that tyrosine kinase activity, which is n...Continue Reading


Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L LeytonP M Saling
Jun 1, 1992·Archives of Biochemistry and Biophysics·P S Chaudhry, E R Casillas
Jan 9, 1992·Biochimica Et Biophysica Acta·G Berruti, S Porzio
Nov 1, 1991·Journal of Reproduction and Fertility·M F Thomson, G J Wishart
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R RotemZ Naor
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Mar 1, 1989·Annals of Clinical Biochemistry·T KobayashiH Yamamura
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart, K Ashizawa
Feb 1, 1981·Fertility and Sterility·D F Katz, J W Overstreet

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Apr 25, 2000·Biochemical and Biophysical Research Communications·O J D'CruzF M Uckun
Oct 11, 2007·Molecular Reproduction and Development·Louis-Jean Bordeleau, Pierre Leclerc
Jan 25, 2006·Molecular Reproduction and Development·Claudia LalancettePierre Leclerc

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