Effects of tyrosol and farnesol on Candida albicans biofilm

Molecular Medicine Reports
Sarra SebaaPhilippe Courtois

Abstract

The present in vitro study examined the effects of the quorum‑sensing molecules farnesol and tyrosol on the development of Candida albicans biofilm in order to elucidate their role as novel adjuvants in oral hygiene. The investigation was conducted in C. albicans ATCC 10231 and C. albicans isolates from dentures and was performed in flat‑bottomed 96‑well polystyrene plates. Yeast growth and their capacity to form biofilms were evaluated following 24 and 48 h incubations at 37˚C in Sabouraud broth supplemented with 0.001‑3 mM farnesol and/or 1‑20 mM tyrosol. Yeast growth was assessed by turbidimetry and biofilms were quantitated by crystal violet staining, under aerobic and anaerobic conditions. The viability of the fungal cells was controlled by the culture of planktonic cells and by examination of the biofilms using fluorescence microscopy following staining with fluorescein diacetate and ethidium bromide. Farnesol at 3 mM exerted a stronger action when added at the beginning of biofilm formation (>50% inhibition) than when added to preformed biofilms (<10% inhibition). Similarly, tyrosol at 20 mM had a greater effect on biofilm formation (>80% inhibition) than on preformed biofilms (<40% inhibition). Despite significant reduc...Continue Reading

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Citations

Aug 11, 2020·World Journal of Microbiology & Biotechnology·Giacomo ZaraSeverino Zara
Dec 3, 2020·Frontiers in Cellular and Infection Microbiology·Tânia GrainhaMaria Olívia Pereira
Nov 1, 2020·International Journal of Molecular Sciences·Yves Combarnous, Thi Mong Diep Nguyen
May 18, 2021·Frontiers in Microbiology·Tanu AtriwalMohammad Abid

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