Effects of unfractionated heparin and rivaroxaban on the expression of heparanase and fibroblast growth factor 2 in human osteoblasts

Molecular Medicine Reports
Jingjun XiaDuanyang Wang

Abstract

Long-term treatment with anticoagulants may contribute to osteoporosis. Although unfractionated heparin and rivaroxaban have adverse effects on bone microstructure and function in adult rats, the underlying mechanism remains to be elucidated. Heparanase (HPSE) and fibroblast growth factor (FGF)2 are important signals in bone formation and fracture healing. Therefore, the present study was designed to investigate the effects of unfractionated heparin and rivaroxaban on the expression of HPSE and FGF2 in human osteoblasts. Human osteoblasts were treated with unfractionated heparin (0.5-50 IU/ml) or rivaroxaban (0.13‑13 µg/ml) for different durations. Plasmids encoding HPSE and FGF2 were transfected into osteoblasts, and cell viability was assessed using MTT assays, with mRNA and protein expression levels determined using reverse transcription‑quantitative polymerase chain reaction and western blot analyses, respectively. Osteoblast growth was significantly inhibited by treatment with unfractionated heparin (50 IU/ml) or rivaroxaban (13 µg/ml). Unfractionated heparin alone significantly inhibited the expression of HPSE and FGF2, whereas rivaroxaban inhibited the expression of FGF2 without affecting that of HPSE. Furthermore, the o...Continue Reading

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Citations

Jul 22, 2020·Geriatrics & Gerontology International·Eiji Kose, Hidetaka Wakabayashi
Apr 26, 2020·Journal of the Chinese Medical Association : JCMA·Li-Te LinPeng-Hui Wang
Mar 3, 2020·Frontiers in Immunology·Deniz RagipogluVerena Fischer
Oct 9, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xinhong LiuXin Zhao

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