Effects of urea on M4-lactate dehydrogenase from elasmobranchs and urea-accumulating Australian desert frogs

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
C J FueryM Guppy

Abstract

We measured the effect of urea on M4-lactate dehydrogenase (M4-LDH) from elasmobranchs and Australian desert frogs (urea accumulators) and from two animals that do not accumulate urea, the axolotl and the rabbit. An analysis of the effect of urea on the Kd(NADH), V, V/K(m(prr)) and V/K(m(NADH)) shows that in all cases the major effect of urea was on the binding of pyruvate, which fits with data in the literature that show that urea acts as a competitive inhibitor of LDH. The characteristics of the elasmobranch enzymes are consistent with a proposed adaptation model, but the situation for the enzymes from the aestivating frogs is equivocal. Urea (400 mM) had less effect on the K(m(prr)) of M4-LDH from the urea accumulators than it did on the non-accumulators, suggesting a general adaptation and that the enzyme produced by the aestivating frogs (urea accumulators) is kinetically different from that of non-aestivating frogs (non-accumulators). A new approach is used to characterize the overall pattern of adaptation to urea. The pattern is similar in an enzyme from an elasmobranch and an aestivating frog despite the temporary presence of urea in the latter and the phylogenetic difference between these animals.

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Citations

Jul 31, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Timothy J MuirRichard E Lee
Nov 22, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kenneth B Storey
Sep 10, 2009·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Timothy J MuirRichard E Lee
Dec 15, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amanda Southwood Williard, Leigh Anne Harden
Feb 15, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Timothy J MuirRichard E Lee
Jan 31, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kyra J Cowan, Kenneth B Storey
Dec 31, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Amjad A KhanArshad H Rahmani

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