Effects of uridine, isomatitol and 4-thiouridine on in vitro cell adhesion and in vivo effects of 4-thiouridine in a lung inflammation model

International Immunopharmacology
S Uppugunduri, Chamilly Gautam

Abstract

Since leukocyte adhesion to endothelial cells is crucial for extravasation of leukocytes to sites of inflammation, inhibition of cell-cell adhesion has been suggested as a means to achieve selective modulation of the immune system. We have, using a static in vitro adhesion assay involving adhesion of granulocytes to tumor necrosis factor alpha (TNFalpha)-stimulated human umbilical vein endothelial cells (HUVEC), found three substances--uridine, isomaltitol and 4-thiouridine-that, independently and significantly, reduced leukocyte adhesion by approximately 30-65%. 4-Thiouridine was also tested in an in vivo model of Sephadex (SDX)-induced lung inflammation with Sprague-Dawley rats. Intratracheal instillation of Sephadex (5 mg/kg) alone resulted in a dramatic increase in lung edema and total leukocyte count after 24 h. A differential count of bronchoalveolar lavage (BAL) cells indicated an increased influx of macrophages, eosinophils and neutrophils. Co-administration of 4-thiouridine significantly reduced lung edema by 38%. There was also a significant reduction of the total leukocyte count by 58%. The differential leukocyte count indicated that eosinophil influx alone was reduced by 70%. After Sephadex challenge, we found eleva...Continue Reading

References

Jan 1, 1986·International Archives of Allergy and Applied Immunology·S M LaycockB A Spicer
Jun 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·R S Walls, P B Beeson
May 1, 1996·American Journal of Respiratory Cell and Molecular Biology·P R GaterL M Renzetti
Mar 15, 1996·Journal of Medicinal Chemistry·H OhmotoA Hasegawa
Jun 1, 1996·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·R KleemannF Hartig
Nov 1, 1995·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·N BuyssensA Herman
Dec 15, 1996·The Journal of Clinical Investigation·B J HickeD Parma
Jul 1, 1997·The Journal of Allergy and Clinical Immunology·W R CatapaniE Mendes
Aug 1, 1997·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·S MatsubaraK Naito
Jun 24, 1999·Cytokine & Growth Factor Reviews·A Meager
Nov 7, 1999·The Journal of Allergy and Clinical Immunology·A J Wardlaw
Dec 14, 1999·The American Journal of Pathology·R F GuoM M Shi
Apr 1, 2000·Australian Family Physician·J Burdon
Apr 26, 2000·The European Respiratory Journal·F FinsnesG Christensen
Feb 15, 2001·The New England Journal of Medicine·W W Busse, R F Lemanske
Feb 27, 1965·British Medical Journal·D M BECROFT, L I PHILLIPS

❮ Previous
Next ❯

Citations

Oct 30, 2015·PloS One·Sudeep Chenna NarendraSrinivas Uppugunduri
May 12, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T MüllerM Idzko
Jun 22, 2017·Scientific Reports·Manish Kumar JeengarSrinivas Uppugunduri
Jun 16, 2007·International Immunopharmacology·C EvaldssonS Uppugunduri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.