PMID: 22580532May 15, 2012Paper

Effects of valproic acid and levetiracetam on viability and cell cycle regulatory genes expression in the OVCAR-3 cell line

Pharmacological Reports : PR
Patrycja KwiecińskaEwa L Gregoraszczuk

Abstract

Concentration- and time-dependent effects of two antiepileptic drugs (AEDs), levetiracetam (LEV) and valproic acid (VPA), on proliferation, cytotoxicity and expression of cell cycle regulatory genes were investigated in a human ovarian cancer cell line, OVCAR-3. Cells were cultured with VPA or LEV, at concentrations between 100 μM and 10 mM. Cell proliferation was determined by alamarBlue and BrdU incorporation assays; cytotoxic effects by tetrazolium hydroxide (XTT), acid phosphatase (AP) and lactate dehydrogenase (LDH) assays. Expression of cell cycle regulatory genes was determined by real-time PCR. Exposure to VPA caused a concentration- and time-dependent decrease in cell proliferation (alamarBlue and BrdU incorporation assays), cytotoxic effects above 2.5 mM (XTT and AP assays) and modulated expression of genes primarily responsible for cell cycle arrest in G(1) phase. Cell proliferation was unaffected by exposure to LEV for 24 h and 120 h (alamarBlue assay), but increased when exposed to LEV for 72 h and 168 h, at concentrations from 250 μM to 1 mM. The BrdU incorporation assay showed no effect of LEV on cell proliferation. LEV was cytotoxic at higher concentrations (AP assay), but modulation in expression of cell cycle ...Continue Reading

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Citations

Jul 21, 2012·Pharmacological Reports : PR·Patrycja KwiecińskaEwa Lucja Gregoraszczuk
May 6, 2015·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Angelija ValančiūtėDonatas Stakišaitis
Nov 10, 2013·Toxicology Letters·Patrycja KwiecińskaEwa Łucja Gregoraszczuk
Oct 26, 2016·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Patrycja KwiecińskaEwa Lucja Gregoraszczuk
May 17, 2018·Frontiers in Cellular Neuroscience·Keren AsrafSigal Fleisher-Berkovich
Jun 19, 2019·Dose-response : a Publication of International Hormesis Society·Milda JuknevičienėDonatas Stakišaitis
Jun 8, 2017·Journal of Experimental & Clinical Cancer Research : CR·Ambra RizzoEmilio Ciusani

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