Effects of varicocele upon the expression of apoptosis-related proteins

Andrologia
F-W ChangG-J Wu

Abstract

Varicocele-associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis-related proteins (the typical protein-dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis-related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer-assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis-related protein activation including caspase-3, poly-ACP-ribose polymerase (PARP), the Bcl-2 family (Bcl-2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase-3 and Bcl-2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele-afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with v...Continue Reading

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Citations

Sep 10, 2011·Journal of Assisted Reproduction and Genetics·Monis Bilal ShamsiRima Dada
Mar 24, 2011·Current Opinion in Obstetrics & Gynecology·Pierre NevouxFrançois Marcelli
Nov 23, 2013·International Urology and Nephrology·Ljubinka Jankovic Velickovic, Vladisav Stefanovic
Aug 27, 2011·Fertility and Sterility·Adel ZalataTaymour Mostafa
Nov 29, 2013·Andrology·V A BozhedomovG T Sukhikh
Jul 26, 2013·Spermatogenesis·Ciler Celik-Ozenci, Arda Tasatargil
Oct 12, 2012·Oxidative Medicine and Cellular Longevity·Juan G ReyesRicardo D Moreno
Jun 27, 2019·Cells·Joana Vieira SilvaMargarida Fardilha
May 6, 2020·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamAshok Agarwal
Aug 30, 2017·Molecular Medicine Reports·Shao-Ming ZhuFan Cheng

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