Abstract
Varicocele-associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis-related proteins (the typical protein-dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis-related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer-assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis-related protein activation including caspase-3, poly-ACP-ribose polymerase (PARP), the Bcl-2 family (Bcl-2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase-3 and Bcl-2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele-afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with v...Continue Reading
References
Sep 1, 1989·The Journal of Urology·M Goldstein, J F Eid
May 1, 1997·The Journal of Urology·S A SocherA Morgentaler
May 1, 1997·The EMBO Journal·I RodriguezP Vassalli
Dec 9, 1997·Cell·P LiX Wang
Dec 11, 1997·Cell·M G Vander HeidenC B Thompson
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
May 18, 1999·Lancet·J R BowenD M Saunders
Aug 24, 1999·The Biochemical Journal·D D'AmoursG G Poirier
Nov 26, 1999·Human Reproduction·M BonduelleA Van Steirteghem
May 16, 2002·BJU International·Hiroyuki TanakaS Kamidono
Mar 10, 2004·Human Reproduction Update·Tamer M SaidAshok Agarwal
Apr 6, 2004·Journal of Andrology·Chi-Huang ChenGwo-Jang Wu
Oct 7, 2004·Molecular Human Reproduction·S L TaylorS J Beebe
Oct 18, 2005·BJU International·Ja Hyeon KuJae-Seung Paick
Citations
Sep 10, 2011·Journal of Assisted Reproduction and Genetics·Monis Bilal ShamsiRima Dada
Mar 24, 2011·Current Opinion in Obstetrics & Gynecology·Pierre NevouxFrançois Marcelli
Nov 23, 2013·International Urology and Nephrology·Ljubinka Jankovic Velickovic, Vladisav Stefanovic
Aug 27, 2011·Fertility and Sterility·Adel ZalataTaymour Mostafa
Oct 30, 2014·Andrologia·A A Eid, D N Younan
Nov 29, 2013·Andrology·V A BozhedomovG T Sukhikh
Sep 9, 2015·BioMed Research International·Letteria MinutoliHerbert Marini
Jul 26, 2013·Spermatogenesis·Ciler Celik-Ozenci, Arda Tasatargil
Oct 12, 2012·Oxidative Medicine and Cellular Longevity·Juan G ReyesRicardo D Moreno
Oct 26, 2018·Andrologia·Shaghayegh Foroozan-BroojeniMohammad Hossein Nasr-Esfahani
Jul 29, 2014·Reproductive Medicine and Biology·V GentileE Bottini
Jan 23, 2015·Andrologia·M LiangB-K Shi
Jun 27, 2019·Cells·Joana Vieira SilvaMargarida Fardilha
May 6, 2020·International Journal of Molecular Sciences·Manesh Kumar Panner SelvamAshok Agarwal
Nov 28, 2019·BMC Complementary and Alternative Medicine·Keshab Kumar KarnaJong Kwan Park
Aug 30, 2017·Molecular Medicine Reports·Shao-Ming ZhuFan Cheng