Effects of VEGF on the blood-brain barrier disruption caused by hyperosmolarity

Pharmacology
Oak Z ChiHarvey R Weiss

Abstract

This study was performed to test whether disruption of the blood-brain barrier (BBB) caused by hyperosmolarity could be related to vascular endothelial growth factor (VEGF), using anti-VEGF antibody and ciclopirox olamine (CPX), an inducer of VEGF. CPX 50 mg/kg or normal saline was given intraperitoneally to male Wistar rats 18 h before BBB disruption. Two craniotomies were made on the ipsilateral cortex (IC-1 and IC-2) where the BBB would be disrupted, and a third hole was made on the contralateral cortex (CC) to expose the cortices. We applied normal saline (to IC-1 and the CC) or anti-VEGF antibody (to IC-2) for 90 min before BBB disruption with intracarotid injection of 25% mannitol. The degree of BBB disruption was determined by measuring the transfer coefficient (K(i)) of (14)C-alpha-aminoisobutyric acid and the volume of (3)H-dextran distribution. The protein levels of VEGF were determined with Western blot analysis. In the control animals, hyperosmolar mannitol significantly increased (415%) the K(i) in IC-1. The K(i) was attenuated with anti-VEGF antibody application (-28%, p < 0.05). Even though the protein levels of VEGF were strongly increased with CPX pretreatment, this upregulation did not alter the hyperosmolar B...Continue Reading

Citations

Mar 10, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·P M Abdul MuneerJames Haorah
Jul 22, 2014·Critical Care : the Official Journal of the Critical Care Forum·Arkom NongnuchAndrew Davenport
Feb 4, 2012·Seizure : the Journal of the British Epilepsy Association·Gan YouTao Jiang
Aug 21, 2018·Neurologia i neurochirurgia polska·Eric D Goldstein, Anteneh M Feyissa
Jan 9, 2019·Kidney Research and Clinical Practice·Maryam Malek
Mar 22, 2020·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Blanca Fabiola Fajardo-FregosoMónica E Ureña-Guerrero

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