Effects of viral hemorrhagic fever inactivation methods on the performance of rapid diagnostic tests for Plasmodium falciparum

The Journal of Infectious Diseases
M R LoutfyK C Kain

Abstract

Blood samples from patients with viral hemorrhagic fever (VHF) pose a serious risk to laboratory workers. Current contingency plans for VHF samples recommend the use of heat, gamma-irradiation, or Triton X-100 to inactivate samples before handling. Malaria is the most important alternative diagnosis to be excluded in cases of suspected VHF. Interpretation of malaria smears using samples inactivated with these methods is problematic because morphology is altered. The objective of this study was to assess the impact of different inactivation methods on the performance of rapid diagnostic tests for Plasmodium falciparum. Triton X-100 and gamma-irradiation of samples preserved detection. The impact of Triton X-100 inactivation was also "blindly" evaluated using 100 blood samples from febrile travelers. Triton X-100 inactivation of samples did not significantly affect the performance of these tests. This may represent a useful strategy for excluding the diagnosis of falciparum malaria in cases of suspected VHF.

Citations

Feb 19, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Chantal ReuskenMarion Koopmans
Dec 3, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Francesca ColavitaConcetta Castilletti
Sep 30, 2017·The Journal of Infectious Diseases·Jeroen J A van KampenMarion P G Koopmans
Jul 28, 2006·Nature·Paul YagerBernhard H Weigl
Jul 15, 2016·Clinical Microbiology Reviews·M Jana BroadhurstNira R Pollock
Jan 30, 2015·Journal of Clinical Microbiology·Rachel LauAndrea K Boggild
Feb 13, 2016·Journal of Clinical Microbiology·Todd A. CuttsSteven S Theriault
Nov 6, 2018·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M M RemyF Suter-Riniker

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