Effects of vitamin A on in vitro maturation of pre-pubertal mouse spermatogonial stem cells

PloS One
Albanne TraversN Rives

Abstract

Testicular tissue cryopreservation is the only potential option for fertility preservation in pre-pubertal boys exposed to gonadotoxic treatment. Completion of spermatogenesis after in vitro maturation is one of the future uses of harvested testicular tissue. The purpose of the current study was to evaluate the effects of vitamin A on in vitro maturation of fresh and frozen-thawed mouse pre-pubertal spermatogonial stem cells in an organ culture system. Pre-pubertal CD1 mouse fresh testes were cultured for 7 (D7), 9 (D9) and 11 (D11) days using an organ culture system. Basal medium was supplemented with different concentrations of retinol (Re) or retinoic acid (RA) alone or in combination. Seminiferous tubule morphology (tubule diameter, intra-tubular cell type), intra-tubular cell death and proliferation (PCNA antibody) and testosterone level were assessed at D7, D9 and D11. Pre-pubertal mouse testicular tissue were frozen after a soaking temperature performed at -7 °C, -8 °C or -9 °C and after thawing, were cultured for 9 days, using the culture medium preserving the best fresh tissue functionality. Retinoic acid at 10(-6)M and retinol at 3.3.10(-7)M, as well as retinol 10(-6)M are favourable for seminiferous tubule growth, ma...Continue Reading

References

Jul 1, 1987·Endocrinology·C Morales, M D Griswold
Mar 15, 1993·Annals of the New York Academy of Sciences·P O LübeckB M Sandström
Oct 31, 1998·Current Opinion in Immunology·C PatienceR A Weiss
Jul 27, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Gabriel LiveraRené Habert
Dec 25, 2002·Theriogenology·J E ParksM T Kaproth
Feb 1, 2003·Andrologia·V Rouiller-FabreR Habert
Apr 20, 2004·Cryobiology·Erik J WoodsJohn K Critser
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Aug 21, 2004·Human Reproduction Update·Herman TournayeAndré Van Steirteghem
Mar 10, 2005·The American Journal of Clinical Nutrition·Maria Berggren SöderlundPeter Nilsson-Ehle
Oct 8, 2005·Human Reproduction·K KvistC Yding Andersen
Feb 25, 2006·Human Reproduction·V RouletN Dejucq-Rainsford
Mar 8, 2006·Cell and Tissue Research·Gabriel LiveraRené Habert
Mar 15, 2006·International Journal of Hematology·Takehiko OgawaKazuyuki Ohbo
Apr 1, 2006·Science·Josephine BowlesPeter Koopman
Apr 6, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Norbert B GhyselinckManuel Mark
Apr 20, 2006·The Journal of Clinical Endocrinology and Metabolism·Romain LambrotVirginie Rouiller-Fabre
May 3, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Karim NayerniaWolfgang Engel
Aug 24, 2007·Development·Josephine Bowles, Peter Koopman
Sep 26, 2007·BMC Public Health·Rongwang YangZhengyan Zhao
Nov 30, 2007·Journal of Andrology·Jan-Bernd StukenborgStefan Schlatt
Feb 8, 2008·Cell Cycle·Emilie TrautmannGabriel Livera
Jul 4, 2009·Reproductive Biomedicine Online·Jinlian HuaKuldip S Sidhu
Jul 28, 2009·Animal Reproduction Science·Wu-Zi DongZhong-Ying Dou
Oct 2, 2009·Organogenesis·Walter HabelerChristelle Monville

❮ Previous
Next ❯

Citations

Jul 20, 2016·Molecular Human Reproduction·A RedaJ-B Stukenborg
Dec 27, 2016·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Robert E ChapinJan-Bernd Stukenborg
Feb 12, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Gurpreet Manku, Martine Culty
May 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Elham MohammadzadehMarefat Ghaffari Novin
May 23, 2020·Annals of Biomedical Engineering·Justine SaulnierChristine Rondanino
Apr 2, 2021·Cell and Tissue Research·Tanushree PatraMukesh Kumar Gupta

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
xenografting
FCS
biopsies

Software Mentioned

LAS Core
Leica
Leica Application Suite

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.