Effects of vitamin B12 on cell proliferation and cellular alkaline phosphatase activity in human bone marrow stromal osteoprogenitor cells and UMR106 osteoblastic cells

Metabolism: Clinical and Experimental
G S KimC S Park

Abstract

Pernicious anemia has recently been recognized as one of the risk factors for osteoporosis and bone fractures, but the underlying pathophysiologic mechanism is still unknown. To determine whether vitamin B12 has any direct effect on osteoblasts, we studied the effects of vitamin B12 on the proliferation and alkaline phosphatase activity in human bone marrow stromal osteoprogenitor cells (hBMSC) and UMR106 osteoblastic cells. Vitamin B12 at concentrations as low as 10(-12) mol/L significantly stimulated [3H]-thymidine incorporation in both types of cells, but concentrations higher than 10(-12) mol/L did not produce a greater effect. Vitamin B12 in the concentration range from 10(-12) to 10(-8) mol/L concentration-dependently increased alkaline phosphatase activity in both hBMSC and UMR106 cells. Based on these results, we suggest that a suppressed activity of osteoblasts may contribute to osteoporosis and fractures in patients with vitamin B12 deficiency.

Citations

Mar 4, 2003·The Journal of Nutrition·Rosalie A M Dhonukshe-RuttenWija A van Staveren
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katherine L TuckerDouglas P Kiel
May 11, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Rosalie A M Dhonukshe-RuttenWija A van Staveren
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·S OzdemM Herrmann
Apr 14, 2009·Calcified Tissue International·Bart L T VaesWilma T Steegenga
Mar 2, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Thomas P VacekNeetu Tyagi
May 29, 2015·Current Osteoporosis Reports·Regan L Bailey, Janneke P van Wijngaarden
May 12, 2015·Nutrients·Zhaoli Dai, Woon-Puay Koh
Feb 22, 2017·World Journal of Gastroenterology : WJG·Federica CavalcoliSara Massironi
Aug 19, 2007·Journal of Asian Natural Products Research·Xu-Juan YangXin-Sheng Yao
Aug 8, 2008·Digestive Diseases and Sciences·Adriana M KakehasiAlfredo J A Barbosa
Oct 5, 2011·Nutrition Reviews·Hala Ahmadieh, Asma Arabi
Apr 12, 2015·Scandinavian Journal of Gastroenterology·Michelle ClarkeHelene McNulty
May 1, 2008·Expert Review of Endocrinology & Metabolism·Wolfgang Herrmann, Markus Herrmann
Oct 13, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Wolfgang HerrmannMarkus Herrmann
Jul 16, 2010·Food and Nutrition Bulletin·Lindsay H AllenGilbert S Omenn
May 31, 2013·The American Journal of Clinical Nutrition·Denise H M HeppeVincent W V Jaddoe
Dec 15, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Z DaiW-P Koh
Oct 11, 2007·Current Osteoporosis Reports·Robert R McLean, Marian T Hannan
Feb 24, 2007·Digestive Diseases and Sciences·Sebahat OzdemReha Artan
Feb 28, 2014·The Proceedings of the Nutrition Society·M ClarkeH McNulty
Mar 20, 2013·Journal of Nutrition and Metabolism·J P van WijngaardenC P G M de Groot
Sep 17, 2014·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Pooneh SalariFarideh Razi
Mar 4, 2011·The Journal of Nutrition, Health & Aging·W Chatthanawaree
Dec 15, 2017·Revista da Associação Médica Brasileira·Luciana Leal Gomes de MacêdoBetania de Jesus E Silva de Almendra Freitas
Nov 17, 2020·Biomedical Engineering Letters·Sayeed FarzanfarBabak Shekarchi
Aug 18, 2020·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Sadaf Oliai AraghiNathalie van der Velde
Mar 4, 2021·Acta Biomaterialia·Susmita BoseDishary Banerjee
Aug 8, 2007·Bone·Markus HerrmannWolfgang Herrmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.