Effects of vitamin D on inflammatory and oxidative stress responses of human bronchial epithelial cells exposed to particulate matter.

PloS One
Paul E PfefferCatherine M Hawrylowicz

Abstract

Particulate matter (PM) pollutant exposure, which induces oxidative stress and inflammation, and vitamin D insufficiency, which compromises immune regulation, are detrimental in asthma. Mechanistic cell culture experiments were undertaken to ascertain whether vitamin D abrogates PM-induced inflammatory responses of human bronchial epithelial cells (HBECs) through enhancement of antioxidant pathways. Transcriptome analysis, PCR and ELISA were undertaken to delineate markers of inflammation and oxidative stress; with comparison of expression in primary HBECs from healthy and asthmatic donors cultured with reference urban PM in the presence/absence of vitamin D. Transcriptome analysis identified over 500 genes significantly perturbed by PM-stimulation, including multiple pro-inflammatory cytokines. Vitamin D altered expression of a subset of these PM-induced genes, including suppressing IL6. Addition of vitamin D suppressed PM-stimulated IL-6 production, although to significantly greater extent in healthy versus asthmatic donor cultures. Vitamin D also differentially affected PM-stimulated GM-CSF, with suppression in healthy HBECs and enhancement in asthmatic cultures. Vitamin D increased HBEC expression of the antioxidant pathway...Continue Reading

References

Mar 1, 1988·The Journal of Experimental Medicine·M LotzD A Carson
Jun 1, 1997·American Journal of Respiratory and Critical Care Medicine·J K ShuteR Djukanović
Nov 26, 1999·The Journal of Nutrition·C M RohdeH F DeLuca
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·R W AtkinsonK Katsouyanni
Jan 18, 2003·Science·Chandrashekhar Pasare, Ruslan Medzhitov
Dec 24, 2003·In Vitro Cellular & Developmental Biology. Animal·Kyle D Kent, Joshua A Bomser
Dec 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Ning LiAndre E Nel
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bertram BleckJoan Reibman
Nov 25, 2006·The New England Journal of Medicine·Waltraud EderErika von Mutius
Mar 9, 2007·The American Journal of Clinical Nutrition·Elina Hyppönen, Chris Power
Sep 22, 2007·The Journal of Clinical Investigation·Gökhan M MutluG R Scott Budinger
Mar 19, 2008·International Journal of Cancer. Journal International Du Cancer·Bo-Ying BaoYi-Fen Lee
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sif HansdottirGary W Hunninghake
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sif HansdottirGary W Hunninghake
Apr 10, 2010·American Journal of Respiratory Cell and Molecular Biology·Takashi KidoStephan F Van Eeden
Jun 22, 2010·Journal of Cellular Biochemistry·Xinjian PengRajendra G Mehta
Nov 26, 2010·Current Allergy and Asthma Reports·Emma S Chambers, Catherine M Hawrylowicz
Mar 23, 2011·Respiratory Research·Christian HerrRobert Bals
Jun 1, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F J Kelly, J C Fussell
Jun 21, 2011·Immunological Reviews·Stephen T Holgate
Sep 13, 2011·Lancet·Manuel A R FerreiraUNKNOWN Australian Asthma Genetics Consortium
Nov 19, 2011·Scandinavian Journal of Medicine & Science in Sports·E C GomesV Stone
Dec 6, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anne SturrockRobert Paine
Dec 29, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Andrew J GhioMichael C Madden
Feb 4, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yong ZhangElena Goleva
Mar 21, 2012·IUBMB Life·Robert C Stanton
Jun 8, 2012·Respirology : Official Journal of the Asian Pacific Society of Respirology·Alison M GowersRobert L Maynard
Jun 9, 2012·The Journal of Pediatrics·Pilar Codoñer-FranchEulalia Alonso-Iglesias
Jan 31, 2013·The Journal of Allergy and Clinical Immunology·Peter J Barnes
May 15, 2013·Molecular Immunology·Li ZuoKatherine S Salisbury
Sep 26, 2013·The Journal of Allergy and Clinical Immunology·Eric B BrandtGurjit K Khurana Hershey
Apr 18, 2014·Annals of the New York Academy of Sciences·Elizabeth H MannCatherine M Hawrylowicz
Nov 15, 2014·American Journal of Respiratory and Critical Care Medicine·Franziska RosserJuan C Celedón
Dec 3, 2014·The Journal of Allergy and Clinical Immunology·Rachel L Miller, David B Peden

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Citations

Jan 5, 2019·Current Opinion in Allergy and Clinical Immunology·Augusto A Litonjua
Mar 12, 2019·Canadian Journal of Physiology and Pharmacology·Nourelhuda A MohammedDoaa M Abdullah
Nov 15, 2019·Current Opinion in Pulmonary Medicine·Annelies BontinckGuy Joos
Aug 29, 2020·Reviews in Medical Virology·Majid Teymoori-Rad, Sayed Mahdi Marashi
Jun 14, 2019·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Béla BükiYunxia Wang Lundberg
Apr 15, 2019·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Tobore Onojighofia Tobore
Oct 2, 2020·Current Opinion in Clinical Nutrition and Metabolic Care·Emanuele CeredaRiccardo Caccialanza
Oct 1, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·León FerderWalter Manucha
Mar 7, 2021·International Journal of Molecular Sciences·Luiza Ayumi Nishiyama MimuraAlexandrina Sartori
Jun 10, 2019·The Journal of Allergy and Clinical Immunology·Ryan D HuffJeremy A Hirota

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
biopsy
ELISA
PCR
nuclear translocation
lavage

Software Mentioned

Viia
GenomeStudio
GraphPad Prism
GenomicsSuite

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