Effects of Vitamin D Receptor Knockout and Vitamin D Deficiency on Corneal Epithelial Wound Healing and Nerve Density in Diabetic Mice.

Diabetes
Xiaowen LuMitchell A Watsky

Abstract

Diabetic keratopathy occurs in ∼70% of all people with diabetes. This study was designed to examine the effects of vitamin D receptor knockout (VDR-/-) and vitamin D deficiency (VDD) on corneal epithelial wound healing and nerve density in diabetic mice. Diabetes was induced using the low-dose streptozotocin method. Corneal epithelial wounds were created using an Algerbrush, and wound healing was monitored over time. Corneal nerve density was measured in unwounded mice. VDR-/- and VDD diabetic mice (diabetic for 8 and 20 weeks, respectively) had slower healing ratios than wild-type diabetic mice. VDR-/- and VDD diabetic mice also showed significantly decreased nerve density. Reduced wound healing ratios and nerve densities were not fully rescued by a supplemental diet rich in calcium, lactose, and phosphate. We conclude that VDR-/- and VDD significantly reduce both corneal epithelial wound healing and nerve density in diabetic mice. Because the supplemental diet did not rescue wound healing or nerve density, these effects are likely not specifically related to hypocalcemia. This work supports the hypothesis that low vitamin D levels can exacerbate preexisting ophthalmic conditions, such as diabetes.

References

Jan 1, 1991·Journal of Neuroscience Research·D WionP Brachet
Mar 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·U KollenkirchenM R Walters
Aug 15, 1980·Science·A W NormanG M Grodsky
Mar 1, 1984·The Journal of Clinical Investigation·S Kadowaki, A W Norman
Jun 1, 2001·The Journal of Nutrition·L E Johnson, H F DeLuca
Mar 15, 2002·Trends in Endocrinology and Metabolism : TEM·Emmanuel GarcionDidier Wion
Apr 17, 2003·Experimental Eye Research·Linda J MüllerTimo M T Tervo
Apr 25, 2003·Neuroscience·D EylesF Feron
Jun 26, 2003·Experimental Eye Research·Helline B PazIlene K Gipson
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erik J SuuronenMay Griffith
Mar 15, 2005·Brain Research Bulletin·F FéronD W Eyles
Nov 26, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·P PozzilliUNKNOWN IMDIAB group
Mar 21, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Martin HewisonJohn S Adams
Oct 27, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Juan KongYan Chun Li
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Apr 12, 2011·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Nathan Efron
Jun 28, 2011·Diabetic Medicine : a Journal of the British Diabetic Association·M TavakoliR A Malik
Aug 4, 2011·American Journal of Physiology. Endocrinology and Metabolism·Isamu YamakawaHiroshi Kimura
Feb 14, 2012·Ophthalmology·Jiucheng He, Haydee E P Bazan
Mar 5, 2013·Clinical Endocrinology·Sheena KayaniyilAnthony J Hanley
Mar 28, 2013·The American Journal of the Medical Sciences·Jennifer ThreattKathie Hermayer
May 7, 2013·Experimental Eye Research·Amanda N FrenchKathryn A Rose
Aug 27, 2013·Trends in Endocrinology and Metabolism : TEM·Tom L Van BelleChantal Mathieu
Dec 18, 2013·Investigative Ophthalmology & Visual Science·Gerard A Lutty
Apr 8, 2014·Nature Reviews. Cancer·David FeldmanBrian J Feldman
Jul 26, 2014·Investigative Ophthalmology & Visual Science·Rodolfo A ElizondoMitchell A Watsky
Feb 26, 2015·Investigative Ophthalmology & Visual Science·Yong-Soo ByunChoun-Ki Joo
Mar 1, 2015·Experimental Eye Research·Rose Y Reins, Alison M McDermott
Mar 7, 2015·Nature Reviews. Drug Discovery·Arndt A SchmitzKhusru Asadullah
Aug 19, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Yuko OdaDaniel D Bikle
Sep 9, 2015·Investigative Ophthalmology & Visual Science·Xiaowen LuMitchell A Watsky
Apr 2, 2016·PloS One·Rose Y ReinsAlison M McDermott
Apr 12, 2016·The Journal of Clinical Investigation·Nan GaoFu-Shin Yu
May 25, 2016·Diabetes·Stephanie R SisleyDarleen A Sandoval

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