Effects of volatile anaesthetics on spontaneous action potential firing of cerebellar Purkinje cells in vitro do not follow the Meyer-Overton rule

British Journal of Anaesthesia
B AntkowiakK Kirschfeld

Abstract

We have investigated in rat brain slices the effects of the volatile anaesthetics enflurane, isoflurane and halothane on spontaneous discharge patterns and mean firing rates of cerebellar Purkinje cells. In the absence of these anaesthetics, Purkinje cells fired bursts of action potentials separated by quiescent periods lasting less than 2 s. Mean discharge rates were 10.8 (SEM 0.4) Hz at 23 +/- 1 degrees C and 25.6 (1.2) Hz at 35 +/- 1 degrees C. The agents exhibited qualitatively different effects when applied at concentrations corresponding to 1-3 MAC. Enflurane markedly lengthened burst and inter-burst durations. Isoflurane acted in a similar manner, but effects were less pronounced. In contrast with isoflurane and enflurane, halothane shortened burst durations. At concentrations corresponding to 1-1.5 MAC, halothane, isoflurane and enflurane significantly depressed action potential firing by 15-30% (P < 0.05). Enflurane 1.2 mmol litre-1 (2.0 MAC), isoflurane 0.9 mmol litre-1 (2.8 MAC) and halothane 0.9 mmol litre-1 (3.8 MAC) depressed spontaneous spike rates by 50%. The changes in discharge patterns and the concentration-dependent decrease in the firing rates were similar at 23 +/- 1 degrees C and 35 +/- 1 degrees C. In su...Continue Reading

Citations

May 26, 2007·Anesthesiology·Christian GrasshoffBernd Antkowiak
Nov 4, 2005·Journal of Neurophysiology·Jacob EngelmannKirsty Grant
Nov 20, 2008·Biological Research for Nursing·Henry C Talley VBrad Roper
Nov 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·B Molaee-ArdekaniE Wodey
Aug 14, 2002·British Journal of Anaesthesia·H A Nash
Feb 26, 2019·Nano Letters·Linlin LuXiaojie Duan

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