Abstract
Cynanchum paniculatum (Bge.) Kitag. (CP) is an important medicinal herb used in Chinese herbal medicine, with a variety of biological activities including anticancer property. In this study, we explored the water extract of CP, for its anticancer effects against breast cancer cells with different mutation types. Cells were grouped as untreated (Control); CP direct treatment (dir-CP); Conditioned medium from CP treated (sup-CP), and untreated cells (sup-Control). Effects of dir-CP and sup-CP were compared to corresponding untreated cells on cytotoxicity, cell migration, and protein expression (cleaved caspase-3, caspase-9, and MMP-2 and 9). CP treatment showed time-dependent decrease in cell number of MDA-MB-231 and SK-Br-3 (both ER(-) PR(-)), while the decrease in cell number was not as significant in MCF-7 and ZR-75-1 cells (both ER(+) PR(+)). sup-CP treatment inhibited the cell migration of MDA-MB-231 and MCF-7 (Her2(-)) in a 24 h scratch assay. Our data suggested that ER(-) PR(-) cells are more sensitive to the CP in terms of direct cytotoxicity, which is not regulated by caspase-3. CP inhibited the migration of the two Her2(-) cells, and this correlated with MMP-2 regulation. The migration of ER(-) PR(-) cells was more sens...Continue Reading
References
Jun 1, 1975·Biochemical Medicine·A B MakarT R Tephly
Feb 7, 2006·Cancer Letters·Rita Nahta, Francisco J Esteva
Aug 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rebecca DentSteven A Narod
Feb 10, 2011·The Oncologist·Clifford A Hudis, Luca Gianni
May 4, 2012·Archives of Pharmacal Research·Jin Bae WeonChoong Je Ma
Nov 7, 2012·Steroids·Jin-Qian YuHai-Lin Qin
Sep 21, 2013·Carbohydrate Research·Chung Sub KimKang Ro Lee
Apr 1, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jiangxia CaiYalin Dong
Apr 23, 2014·PloS One·Eliana SpiliotiParaskevi Moutsatsou
Aug 22, 2014·Biomolecules & Therapeutics·Yetao OuShaobo Zhou
Mar 12, 2015·Molecular Medicine Reports·Weipeng ZhangYalin Dong
Oct 16, 2015·Journal of Asian Natural Products Research·Dan ZhaoYue-Hu Pei
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing WuRuichen Guo
Jul 7, 2016·Fitoterapia·Dan ZhaoYuehu Pei
Aug 16, 2017·Journal of Ethnopharmacology·Josiane HilbigJane Mara Block
Sep 16, 2017·Mediators of Inflammation·Junko TsuchidaToshifumi Wakai
Oct 27, 2017·Advanced Pharmaceutical Bulletin·Behzad MansooriBehzad Baradaran
Nov 22, 2017·Journal of Cancer·Xiaofeng DaiJia Li
May 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lu HanChangcai Bai
May 31, 2018·Cancer Biotherapy & Radiopharmaceuticals·Roland O SaaheneDezhi Pang
Sep 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Charinya KhamphukdeeAnake Kijjoa
Apr 28, 2019·Natural Products and Bioprospecting·Hai-Li YuXiao-Jiang Hao
May 9, 2019·International Journal of Molecular Sciences·Peng ZengHu Chen
Aug 10, 2019·Cancers·Priyanka BhatejaBhuvaneswari Ramaswamy
Jun 26, 2020·Molecular Medicine Reports·Javier Ramírez-RicardoEduardo Perez Salazar
Jul 3, 2020·Breast Cancer Research and Treatment·Vincent WalterMarkus Wallwiener
Aug 11, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Jing-Xian ChenLan Zheng