Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice.

World Journal of Gastroenterology : WJG
Ai-Guang ZhaoJin-Kun Yang

Abstract

To investigate the expression of multiple genes in Chinese jianpi herbal recipe Wei Chang An (WCA) in human gastric cancer cell line SGC-7901. A human gastric adenocarcinoma cell line SGC-7901 grafted onto nude mice was used as the animal model. The mice were randomly divided into 3 groups, one control and the two representing experimental conditions. Animals in the two experimental groups received either WCA over a 34-d period or 5-fluorouracil (5-FU) over 6-d period starting at 8th d after grafting. Control animals received saline on an identical schedule. Animals were killed 41 d after being grafted. The expression profiles in paired WCA treated gastric cancer samples and the N.S. control samples were studied by using a cDNA array representing 14181 cDNA clusters. The alterations in gene expression levels were confirmed by Real-time Quantitative polymerase chain reaction (qPCR). When compared with controls, the average tumor inhibitory rate in WCA group was 44.32% +/- 5.67% and 5-FU 47.04% +/- 11.33% (P < 0.01, respectively). The average labeling index (LI) for PCNA in WCA group and 5-FU group was significantly decreased compared with the control group. Apoptotic index (AI) was significantly increased to 9.72% +/- 4.51% usin...Continue Reading

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Citations

Mar 17, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ai-guang ZhaoJin-yun Yang
Nov 16, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kanjoormana A ManuGautam Sethi
Nov 20, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jae Ho ChoiHye Gwang Jeong

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