PMID: 7541403Jan 1, 1995Paper

Effects of whole blood flow rates on mononuclear cell yields during peripheral blood stem cell collection using Fenwal CS 3000 Plus

Journal of Clinical Apheresis
J S LinMorie A Gertz

Abstract

It is generally assumed that using high whole blood flow rates (WBFR), 80 ml/min, during peripheral blood stem cell (PBSC) collection on the Fenwal CS 3000 Plus blood cell processor will result in higher yields of mononuclear cells (MNC) than using lower WBFR (50 ml/min). To test this assumption, we retrospectively studied 129 PBSC procedures on 17 patients in a multiple myeloma protocol comparing MNC yield, as well as red blood cell (RBC), granulocyte, and platelet (Plt) content, of four average WBFR groups. Standard PBSC procedures were performed using modified procedure 1, interface offset 100, anticoagulant (AC) ratio of 11:1, small volume collection chamber, and a processing time of 4 hours. After correcting for AC volume used, the volume processed was divided by 240 minutes to obtain the average WBFR. WBFRs were separated into 4 groups of 40-49, 50-59, 60-69, and 70-79 ml/min. When compared to the highest flow rate group (70-79 ml/min), the three lower flow rate groups had significantly higher MNC yields of 16.2 +/- 6.9, 13.1 +/- 5.1, and 11.5 +/- 4.7 x 10(9), respectively, as compared to 8.9 +/- 6.1 x 10(9) MNC for the 70-79 ml/min group. There was no significant difference in granulocyte yield which ranged from 1.6 +/- ...Continue Reading

Citations

May 20, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Rainer Moog
Nov 6, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Onder Arslan, Rainer Moog
Mar 21, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Fevzi AltuntasAli Unal
Apr 27, 2012·Journal of Clinical Apheresis·Kambiz Kalantari
Nov 24, 2004·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Rainer Moog
Oct 22, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Sergio GiraltSteven Devine
Apr 12, 2002·Journal of Clinical Apheresis·C D FordL Kelley

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