Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer

Oncology Letters
Wan-Qun ChenHai-Xia Deng

Abstract

Gastric cancer (GC) is one of the most common types of cancer and a leading cause of cancer-associated mortality. MicroRNAs (miRNAs/miRs) are demonstrated to function as oncomiRs or tumor-suppressor-miRs in GC. miR-7 has been identified to be a tumor suppressor of GC by targeting epidermal growth factor receptor (EGFR). In our previous study, Yangzheng Sanjie Decoction (YZSJD), a traditional Chinese formula, was identified to be effective in alleviating the symptoms and even postponing turnover of precancerous lesions. To elucidate the mechanism of YZSJD, the present study evaluated the effects of YZSJD of the GC MKN-45 cell line and investigated the underlying molecular mechanisms using YZSJD-containing serum (YCS). The expression of miR-7 in GC, normal and adjacent tissue samples was examined. The results demonstrated that YCS inhibited proliferation by inducing cell cycle arrest at the S phase, and significantly induced apoptosis compared with the control group. miR-7 was significantly downregulated in GC tissues compared with the matched ones. Using reverse transcription-quantitative polymerase chain reaction and western blot analysis, the expression of miR-7 was inversely associated with EGFR. This indicates that YCS inhib...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 10, 2005·Nature·Jun LuTodd R Golub
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
May 15, 2007·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ai-guang ZhaoJia-xin Qiu
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Dec 27, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M Gordaliza
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Jul 25, 2009·Lancet·Henk H HartgrinkCornelis J H van de Velde
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Mar 17, 2010·Journal of Ethnopharmacology·Min RenHuiqiu Yin
Jan 25, 2011·Surgical Oncology·J WangZ-Q Ma
May 16, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stephen R SmalleyJohn S Macdonald
Jun 23, 2012·Digestive Diseases·Alexander LinkPeter Malfertheiner
Sep 14, 2012·International Journal of Oncology·Wen G JiangMalcolm D Mason
Dec 12, 2012·Journal of Translational Medicine·Mariko MasudaHironobu Sasano
May 4, 2013·Gastroenterology Clinics of North America·Evgeny Yakirevich, Murray B Resnick
Jun 29, 2013·The Journal of Molecular Diagnostics : JMD·Shine Young KimHyung Hoi Kim
Sep 21, 2013·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Florian LordickDavid Ilson
Oct 12, 2013·Digestive Diseases and Sciences·Fuyi TongShenlin Liu
Jan 25, 2014·Expert Reviews in Molecular Medicine·Hua-Hsi WuKuo-Wang Tsai
May 3, 2014·Chinese Journal of Integrative Medicine·Zhao-xiang BianHollie Cheung
Aug 8, 2014·BioMed Research International·Aaron L OomChengfeng Yang
Sep 19, 2014·Case Reports in Oncology·Dong-Hyun LeeJae-Bok Han
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Aug 31, 2017·World Journal of Gastroenterology : WJG·Hai-Xia DengGeng-Xin Chen

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Methods Mentioned

BETA
PCR
electrophoresis
fluorescence-activated cell sorting
fluorescence microscopy
surgical resection

Software Mentioned

Image Lab
Stata

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