Effects of Zhizi Chuanxiong Capsule () on the Abnormal Methylation in Rabbits with Atherosclerosis

Chinese Journal of Integrative Medicine
Qing-Bing ZhouFeng-Qin Xu

Abstract

To investigate the effects of Zhizi Chuanxiong Capsule (ZCC, ) on abnormal DNA methylation in a rabbit model of atherosclerosis (AS). After 1 week of adaptive feeding, 48 New Zealand white rabbits were randomly divided into 4 groups: a control group (n=12) fed with normal diet for 22 weeks; a model group (n=12) fed with high fat diet for 14 weeks followed by 8 weeks of normal diet feeding; a low-dose ZCC group (n=12) fed with high fat diet and low-dose ZCC for 14 weeks, followed by 8 weeks of normal diet and low-dose drug; a high-dose ZCC group (n=12) fed with high fat diet and high-dose drug for 14 weeks, followed by 8 weeks of normal diet and high-dose drug. After 22 weeks of feeding, blood samples were taken from the rabbit ear vein, and the genomic DNA was extracted for methylation immunoprecipitation sequencing (Medip-seq). The aorta tissues were collected for hematoxylin-eosin (HE) staining. Eight rabbits died during the feeding process. HE staining showed that the size of the lipid deposition on vessel wall and atherosclerotic plaque formation were reduced in both low- and high-dose group. The Medip-seq results showed that there were 146 abnormally methylated genes (including both hypermethylated gene and hypomethylated ...Continue Reading

References

Nov 21, 2007·Methods in Molecular Biology·Kiyoko F Aoki-Kinoshita, Minoru Kanehisa
Feb 24, 2009·Biochimica Et Biophysica Acta·Mikko P TurunenSeppo Ylä-Herttuala
Nov 19, 2009·Nucleic Acids Research·UNKNOWN Gene Ontology Consortium
Mar 12, 2015·Trends in Pharmacological Sciences·Concetta SchianoClaudio Napoli
Apr 4, 2016·International Journal of Cardiology·Taulant MukaOscar H Franco
Jul 1, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Jee Yeon KimJei Kim

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Citations

Jul 15, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yan ZhangFengqin Xu

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