Effects of zinc and copper on cadmium uptake by brush border membrane vesicles

Toxicology Letters
T EndoM Sakata

Abstract

The effects of essential metals, zinc (Zn) and copper (Cu), on cadmium (Cd) uptake were investigated in brush border membrane vesicles (BBMV) isolated from the rat renal cortex and LLC-PK1 cells. BBMV were incubated with Cd in the presence or absence of Zn or Cu, and then washed with a chelating agent, EGTA, to remove Cd bound to the outer surface of BBMV. Co-incubation with Zn or Cu decreased Cd accumulation in these BBMV in a concentration-dependent manner. Kinetic analysis of the initial accumulation of Cd suggested that Cd is taken up into rat BBMV via an unsaturable component and a saturable component (K(m) = 13.8 microM, V(max) = 1.44 nmol/mg protein/min), and co-incubation with Zn significantly increased the K(m) of the saturable component without affecting the V(max), whereas Cu significantly increased the K(m)-value and decreased the V(max)-value. Increasing the osmolarity of the incubation medium slightly decreased Cd accumulation in the absence of Zn or Cu, whereas it did not decrease Cd accumulation in the presence of these metals. These results suggest the possibility that, in addition to passive diffusion, Cd is also taken up from the renal brush border membrane via carrier-mediated mechanisms that are inhibited b...Continue Reading

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Citations

Mar 7, 2003·Toxicology and Applied Pharmacology·Rudolfs K Zalups, Sarfaraz Ahmad
Aug 29, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M M Brzóska, J Moniuszko-Jakoniuk
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Jul 7, 2007·Journal of Toxicology and Environmental Health. Part a·Céline Ohayon-CourtèsBéatrice L'Azou
Jan 25, 2005·Nephron. Physiology·Olivier BarbierMarc Cougnon
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HershfinkelI Sekler
Jan 12, 1999·Toxicology and Applied Pharmacology·D A Limaye, Z A Shaikh
Jan 1, 2001·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals· A Karakaya Z Karaaslan Y Duydu B Yücesoy G Oflaz S K Köse
Mar 22, 2016·Metallomics : Integrated Biometal Science·Selvaraj RajakumarVasanthi Nachiappan
Apr 23, 2005·Toxicology and Applied Pharmacology·Christy C Bridges, Rudolfs K Zalups

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