PMID: 7539510Jul 1, 1995Paper

Effects on DNA synthesis and translocation caused by mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase

Journal of Virology
S W Blain, S P Goff

Abstract

To determine the various roles of RNase H in reverse transcription, we generated a panel of mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase based on sequence alignments and the crystal structures of Escherichia coli and human immunodeficiency virus type 1 RNases H (S. W. Blain and S. P. Goff, J. Biol. Chem. 268:23585-23592, 1993). These mutations were introduced into a full-length provirus, and the resulting genomes were tested for infectivity by transient transfection assays or after generation of stable producer lines. Several of the mutant viruses replicated normally, some showed significant delays in infectivity, and others were noninfectious. Virions were collected, and the products of the endogenous reverse transcription reaction were examined to determine which steps might be affected by these mutations. Some mutants left their minus-strand strong-stop DNA in RNA-DNA hybrid form, in a manner similar to that of RNase H null mutants. Some mutants showed increased polymerase pausing. Others were impaired in first-strand translocation, independently of their wild-type ability to degrade genomic RNA, suggesting a new role for RNase H in strand transfer. DNA products synthesized in vivo b...Continue Reading

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Citations

Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wen-Hui ZhangVinay K Pathak
Aug 17, 2006·Journal of Virology·David LimStephen P Goff
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·G GaoS P Goff
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S RajeevanS D Vernon
Aug 6, 2020·Viruses·Eileen A Moran, Susan R Ross
Sep 12, 2002·Expert Opinion on Therapeutic Targets·M L AndréolaS Litvak
Oct 12, 2005·Journal of Molecular Biology·Mark Nils HansonRobert A Bambara

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