Effects on fibrinogen, fibrin, and blood coagulation of proteolytic extracts from fruits of Pseudananas macrodontes, Bromelia balansae, and B. hieronymi (Bromeliaceae) in comparison with bromelain

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
María E ErrastiN O Caffini

Abstract

Extracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. Given the close relationship between hemostasis and inflammation, it is attractive to investigate therapeutic agents capable of modulating both systems. The aim of this work was to study the effect of Pm, Bb, and Bh on fibrin(ogen) and blood coagulation compared with stem bromelain (Bro). Action on fibrinogen was electrophoretically and spectrophotometrically evaluated, fibrinolytic activity was measured both electrophoretically and by the fibrin plate assay, and the effect on blood coagulation was studied by conventional coagulation tests (PT and APPT). All extracts showed the same proteolytic preference for fibrinogen subunits, that is Aα > Bβ, whereas γ was partially hydrolyzed by 100-fold concentration increase. Unlike Bro, cysteine proteases of Pm, Bb, and Bh increased absorbance at 540 nm of fibrinogen solution, suggesting thrombin-like activity, which was time-dependent and reached maximum values at lower concentration. All extracts showed the same proteolytic preference for fibrin subunits; however Pm, Bb, and Bh showed...Continue Reading

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Citations

Oct 30, 2016·Bioengineered· Adivitiya, Yogender Pal Khasa
Nov 28, 2017·Current Medicinal Chemistry·Anastasia V BalakirevaAndrey A Zamyatnin
Dec 21, 2019·Memórias do Instituto Oswaldo Cruz·Ayrles Fb SilvaMarcio V Ramos
Mar 16, 2018·Applied Biochemistry and Biotechnology·María E ErrastiLaura M I López
Nov 5, 2020·Burns : Journal of the International Society for Burn Injuries·Daniel A HofmaennerJan A Plock
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wanqing YangShouying Du

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Methods Mentioned

BETA
electrophoretic profile

Software Mentioned

GraphPad Prism

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