PMID: 41160Jan 1, 1978

Effects on mitochondrial K flux of pH, K concentration, and N-ethyl maleimide

Membrane Biochemistry
J J Diwan, P H Lehrer

Abstract

Based on published evidence that cation transport in mitochondria is not significantly dependent on a membrane potential, it is suggested that the process of mitochondrial cation transport may be nonelectrogenic. These experiments focused on the possibility that K+ flux into rat liver mitochondria may be directly coupled, via an energy-linked carrier mechanism, to OH- influx or H+ efflux. The dependence of the unidirectional K+ influx on the external K+ concentration indicates involvement of a saturable mechanism. Increasing the external pH from 7.0 to 8.0 increases the apparent V max of the K+ influx without significantly altering the apparent Km for K+. The pH dependence is greater in the presence of N-ethyl maleimide, a known inhibitor of the mitochondrial Pi/OH- exchange mechanism. N-Ethyl maleimide decreases the apparent V max at pH 7.0 and increases it at pH 8.0. Evidence indicates that both N-ethyl maleimide and a high external Pi concentration may stimulate the K+ influx at alkaline external pH (8.0) by preventing net exchanges between endogenous Pi and external OH-. An apparent first-order dependence of the K+ influx on the external OH- concentration is observed in the presence of N-ethyl maleimide. These results are c...Continue Reading

References

Apr 1, 1986·Journal of Bioenergetics and Biomembranes·J J DiwanT Haley
Feb 1, 1982·Journal of Bioenergetics and Biomembranes·J J Diwan
Aug 1, 1980·Journal of Bioenergetics and Biomembranes·J J DiwanN O Gonsalves
Apr 1, 1988·Journal of Bioenergetics and Biomembranes·J J DiwanC Moore
Jan 1, 1985·The Journal of Membrane Biology·J J Diwan
Aug 20, 1982·Biochimica Et Biophysica Acta·H S Chang, J J Diwan
May 1, 1980·Biological Reviews of the Cambridge Philosophical Society·H Tedeschi
Jun 12, 1979·Biochemistry·J J DiwanD Aronson
Jan 1, 1987·Biochimica Et Biophysica Acta·J J Diwan
Apr 27, 1979·Biochemical and Biophysical Research Communications·L M Gauthier, J J Diwan

Citations

Mar 20, 1975·Biochimica Et Biophysica Acta·P C LarisR R Chaffee
Dec 1, 1975·FEBS Letters·J J Diwan, H Tedeschi
Jan 1, 1973·The Journal of Membrane Biology·H Rottenberg
Aug 4, 1970·Biochimica Et Biophysica Acta·L E BakeevaV P Skulachev
Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J T Tupper, H Tedeschi
Nov 5, 1974·Biochemistry·H Rottenberg, A Scarpa
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Tedeschi
Jan 23, 1973·Biochemical and Biophysical Research Communications·J J Diwan
Feb 1, 1973·Archives of Biochemistry and Biophysics·S Izzard, H Tedeschi
Jan 18, 1973·Biochimica Et Biophysica Acta·S PapaD Larosa
Jul 1, 1972·Archives of Biochemistry and Biophysics·J J Diwan
Feb 1, 1970·European Journal of Biochemistry·S Massari, G F Azzone
Jan 14, 1969·Biochimica Et Biophysica Acta·E J Harris, B C Pressman
Jun 1, 1969·Archives of Biochemistry and Biophysics·J H Johnson, B C Pressman
Dec 1, 1972·The Journal of Membrane Biology·E J Harris, J A Bangham

Related Concepts

Ethylmaleimide
Maleimides
Dinitrophenols
Cation Transport
Potassium
Mitochondria, Liver
Mitochondria
Antimycin A1
Hydrogen-Ion Concentration
Maleimide

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