Efficacy and Safety of Glycosidic Enzymes for Improved Gene Delivery to the Retina following Intravitreal Injection in Mice

Molecular Therapy. Methods & Clinical Development
Jasmina Cehajic-KapetanovicPaul N Bishop

Abstract

Viral gene delivery is showing great promise for treating retinal disease. Although subretinal vector delivery has mainly been used to date, intravitreal delivery has potential advantages if low retinal transduction efficiency can be overcome. To this end, we investigated the effects of co-injection of glycosaminoglycan-degrading enzymes, singly or in combination, with AAV2 as a method of increasing retinal transduction. Experiments using healthy mice demonstrated that these enzymes enhance retinal transduction. We found that heparinase III produced the greatest individual effect, and this was enhanced further by combination with hyaluronan lyase. In addition, this optimized AAV2-enzyme combination led to a marked improvement in transduction in retinas with advanced retinal degeneration compared with AAV2 alone. Safety studies measuring retinal function by flash electroretinography indicated that retinal function was unaffected in the acute period and at least 12 months after enzyme treatment, whereas pupillometry confirmed that retinal ganglion cell activity was unaffected. Retinal morphology was not altered by the enzyme injection. Collectively these data confirm the efficacy and safety of this intravitreal approach in enhanc...Continue Reading

Citations

Jun 12, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Cleildo P SantanaArmando da Silva-Cunha
Dec 3, 2020·Frontiers in Neuroscience·Michelle E McClementsJasmina Cehajic-Kapetanovic
Mar 6, 2021·Molecular Therapy. Methods & Clinical Development·Elisa MurenuStylianos Michalakis
Apr 18, 2020·Molecular Therapy. Methods & Clinical Development·Sanjar Batirovich MadrakhimovTae Kwann Park

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