Efficacy of a murine-p75-saporin immunotoxin for selective lesions of basal forebrain cholinergic neurons in mice

Neuroscience Letters
Nupur NagJoanne E Berger-Sweeney

Abstract

Selective lesioning of cholinergic neurons in the basal forebrain provides a tool for examining the functional significance of cholinergic loss, which is associated with a number of developmental and neurodegenerative disorders. A new version of an immunotoxin (murine-p75NTR-saporin) was used to produce a selective loss of cholinergic neurons in the adult basal forebrain of the mouse. This new version of the toxin is significantly more potent and selective than a previously developed version. C57Bl/6J mice (n=30) were given 1 microL of either saline or murine-p75NTR-saporin (0.65 microg/microL or 1.3 microg/microL) into the lateral ventricles, and then sacrificed 10-12 days post-surgery for histological analysis. In contrast to results from the previous version of the toxin, survival of the toxin-treated mice was 100% at both doses. A complete loss of cholinergic neurons was seen in the medial septum (MS) with both doses, while a dose-dependent loss of cholinergic neurons was observed in the nucleus basalis magnocellularis (nBM). The lesions were associated with locomotor hypoactivity and anxiolytic-type behavioral effects. These studies describe the efficacy and selectivity of this new version of murine-p75NTR-saporin, which m...Continue Reading

References

Jan 1, 1985·Annals of the New York Academy of Sciences·R T BartusC Flicker
Nov 24, 2001·Neuroscience·C C Wrenn, R G Wiley
Jan 30, 2004·Learning & Memory·Joseph A VuckovichMark G Baxter
Jun 26, 2004·The European Journal of Neuroscience·Christopher L HunterAnn-Charlotte Granholm

❮ Previous
Next ❯

Citations

Jul 30, 2014·Translational Psychiatry·L T LottaN F Schor
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tae KimRobert W McCarley
Mar 14, 2013·Journal of Neuropathology and Experimental Neurology·Juan Jose Ramos-RodriguezMonica Garcia-Alloza
Nov 27, 2010·Behavioural Brain Research·Inge KlinkenbergArjan Blokland
Jul 27, 2021·Frontiers in Neural Circuits·Yuichi TakeuchiAntal Berényi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain & Food Avoidance

Neurons in the basal forebrain play specific roles in regulating feeding. Here are the latest discoveries pertaining to the basal forebrain and food avoidance.

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.